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The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases.
Chow J, Kovacic F, Dall Antonia Y, Krauss U, Fersini F, Schmeisser C, Lauinger B, Bongen P, Pietruszka J, Schmidt M, Menyes I, Bornscheuer UT, Eckstein M, Thum O, Liese A, Mueller-Dieckmann J, Jaeger KE, Streit WR. Chow J, et al. Among authors: kovacic f. PLoS One. 2012;7(10):e47665. doi: 10.1371/journal.pone.0047665. Epub 2012 Oct 24. PLoS One. 2012. PMID: 23112831 Free PMC article.
Probing enzyme promiscuity of SGNH hydrolases.
Leščić Ašler I, Ivić N, Kovačić F, Schell S, Knorr J, Krauss U, Wilhelm S, Kojić-Prodić B, Jaeger KE. Leščić Ašler I, et al. Among authors: kovacic f. Chembiochem. 2010 Oct 18;11(15):2158-67. doi: 10.1002/cbic.201000398. Chembiochem. 2010. PMID: 20931591
Igni18, a novel metallo-hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I: cloning, expression, purification and X-ray analysis.
Kobus S, Perez-Garcia P, Hoeppner A, Holzscheck N, Kovacic F, Streit WR, Jaeger KE, Chow J, Smits SHJ. Kobus S, et al. Among authors: kovacic f. Acta Crystallogr F Struct Biol Commun. 2019 Apr 1;75(Pt 4):307-311. doi: 10.1107/S2053230X19002851. Epub 2019 Apr 2. Acta Crystallogr F Struct Biol Commun. 2019. PMID: 30950832 Free PMC article.
A phospholipase B from Pseudomonas aeruginosa with activity towards endogenous phospholipids affects biofilm assembly.
Weiler AJ, Spitz O, Gudzuhn M, Schott-Verdugo SN, Kamel M, Thiele B, Streit WR, Kedrov A, Schmitt L, Gohlke H, Kovacic F. Weiler AJ, et al. Among authors: kovacic f. Biochim Biophys Acta Mol Cell Biol Lipids. 2022 Apr;1867(4):159101. doi: 10.1016/j.bbalip.2021.159101. Epub 2022 Jan 19. Biochim Biophys Acta Mol Cell Biol Lipids. 2022. PMID: 35063652
Determination of lipolytic enzyme activities.
Jaeger KE, Kovacic F. Jaeger KE, et al. Among authors: kovacic f. Methods Mol Biol. 2014;1149:111-34. doi: 10.1007/978-1-4939-0473-0_12. Methods Mol Biol. 2014. PMID: 24818902
33 results