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Structural plasticity and thermal stability of the histone-like protein from Spiroplasma melliferum are due to phenylalanine insertions into the conservative scaffold.
Timofeev VI, Altukhov DA, Talyzina AA, Agapova YK, Vlaskina AV, Korzhenevskiy DA, Kleymenov SY, Bocharov EV, Rakitina TV. Timofeev VI, et al. Among authors: korzhenevskiy da. J Biomol Struct Dyn. 2018 Dec;36(16):4392-4404. doi: 10.1080/07391102.2017.1417162. Epub 2018 Jan 7. J Biomol Struct Dyn. 2018. PMID: 29283021
Molecular dynamics complemented by site-directed mutagenesis reveals significant difference between the interdomain salt bridge networks stabilizing oligopeptidases B from bacteria and protozoa in their active conformations.
Petrenko DE, Mikhailova AG, Timofeev VI, Agapova YК, Karlinsky DM, Komolov AS, Korzhenevskiy DA, Vlaskina AV, Rumsh LD, Rakitina TV. Petrenko DE, et al. Among authors: korzhenevskiy da. J Biomol Struct Dyn. 2020 Oct;38(16):4868-4882. doi: 10.1080/07391102.2019.1692694. Epub 2019 Nov 25. J Biomol Struct Dyn. 2020. PMID: 31724904
Activity modulation of the oligopeptidase B from Serratia proteamaculans by site-directed mutagenesis of amino acid residues surrounding catalytic triad histidine.
Mikhailova AG, Rakitina TV, Timofeev VI, Karlinsky DM, Korzhenevskiy DA, Agapova YК, Vlaskina AV, Ovchinnikova MV, Gorlenko VA, Rumsh LD. Mikhailova AG, et al. Among authors: korzhenevskiy da. Biochimie. 2017 Aug;139:125-136. doi: 10.1016/j.biochi.2017.05.013. Epub 2017 May 27. Biochimie. 2017. PMID: 28554571
Dodecamers derived from the crystal structure were found in the pre-crystallization solution of the transaminase from the thermophilic bacterium Thermobaculum terrenum by small-angle X-ray scattering.
Marchenkova MA, Konarev PV, Rakitina TV, Timofeev VI, Boikova AS, Dyakova YA, Ilina KB, Korzhenevskiy DA, Yu Nikolaeva A, Pisarevsky YV, Kovalchuk MV. Marchenkova MA, et al. Among authors: korzhenevskiy da. J Biomol Struct Dyn. 2020 Jul;38(10):2939-2944. doi: 10.1080/07391102.2019.1649195. Epub 2019 Aug 8. J Biomol Struct Dyn. 2020. PMID: 31347457
Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the histone-like HU protein from Spiroplasma melliferum KC3.
Boyko K, Gorbacheva M, Rakitina T, Korzhenevskiy D, Vanyushkina A, Kamashev D, Lipkin A, Popov V. Boyko K, et al. Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):24-7. doi: 10.1107/S2053230X14025333. Epub 2015 Jan 1. Acta Crystallogr F Struct Biol Commun. 2015. PMID: 25615963 Free PMC article.
Structure of recombinant prolidase from Thermococcus sibiricus in space group P21221.
Timofeev V, Slutskaya E, Gorbacheva M, Boyko K, Rakitina T, Korzhenevskiy D, Lipkin A, Popov V. Timofeev V, et al. Acta Crystallogr F Struct Biol Commun. 2015 Aug;71(Pt 8):951-7. doi: 10.1107/S2053230X15009498. Epub 2015 Jul 28. Acta Crystallogr F Struct Biol Commun. 2015. PMID: 26249680 Free PMC article.
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