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Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals.
Su Y, Walker JR, Park Y, Smith TP, Liu LX, Hall MP, Labanieh L, Hurst R, Wang DC, Encell LP, Kim N, Zhang F, Kay MA, Casey KM, Majzner RG, Cochran JR, Mackall CL, Kirkland TA, Lin MZ. Su Y, et al. Among authors: kirkland ta. Nat Methods. 2020 Aug;17(8):852-860. doi: 10.1038/s41592-020-0889-6. Epub 2020 Jul 13. Nat Methods. 2020. PMID: 32661427 Free PMC article.
Target engagement and drug residence time can be observed in living cells with BRET.
Robers MB, Dart ML, Woodroofe CC, Zimprich CA, Kirkland TA, Machleidt T, Kupcho KR, Levin S, Hartnett JR, Zimmerman K, Niles AL, Ohana RF, Daniels DL, Slater M, Wood MG, Cong M, Cheng YQ, Wood KV. Robers MB, et al. Among authors: kirkland ta. Nat Commun. 2015 Dec 3;6:10091. doi: 10.1038/ncomms10091. Nat Commun. 2015. PMID: 26631872 Free PMC article.
Three Efficient Methods for Preparation of Coelenterazine Analogues.
Shakhmin A, Hall MP, Walker JR, Machleidt T, Binkowski BF, Wood KV, Kirkland TA. Shakhmin A, et al. Among authors: kirkland ta. Chemistry. 2016 Jul 18;22(30):10369-75. doi: 10.1002/chem.201601111. Epub 2016 Jun 15. Chemistry. 2016. PMID: 27305599
The luminescent HiBiT peptide enables selective quantitation of G protein-coupled receptor ligand engagement and internalization in living cells.
Boursier ME, Levin S, Zimmerman K, Machleidt T, Hurst R, Butler BL, Eggers CT, Kirkland TA, Wood KV, Friedman Ohana R. Boursier ME, et al. Among authors: kirkland ta. J Biol Chem. 2020 Apr 10;295(15):5124-5135. doi: 10.1074/jbc.RA119.011952. Epub 2020 Feb 27. J Biol Chem. 2020. PMID: 32107310 Free PMC article.
40 results