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New human immunodeficiency virus, type 1 reverse transcriptase (HIV-1 RT) mutants with increased fidelity of DNA synthesis. Accuracy, template binding, and processivity.
Kim B, Ayran JC, Sagar SG, Adman ET, Fuller SM, Tran NH, Horrigan J. Kim B, et al. J Biol Chem. 1999 Sep 24;274(39):27666-73. doi: 10.1074/jbc.274.39.27666. J Biol Chem. 1999. PMID: 10488107 Free article.
Amino acid substitution at only three residues, including Asp-76 (Kim, B., Hathaway, T. R., and Loeb, L. A. (1996) Biochemistry 37, 5831-5839), is known to increase fidelity. ...Interestingly, R78A RT exhibited 6- to 8-fold decreases in binding affinity (K(d)) for R …
Amino acid substitution at only three residues, including Asp-76 (Kim, B., Hathaway, T. R., and Loeb, L. A. (1996) Biochemistr …
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