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Perdeuteration, large crystal growth and neutron data collection of Leishmania mexicana triose-phosphate isomerase E65Q variant.
Acta Crystallogr F Struct Biol Commun. 2019 Apr 1;75(Pt 4):260-269. doi: 10.1107/S2053230X19001882. Epub 2019 Apr 2.
Acta Crystallogr F Struct Biol Commun. 2019.
PMID: 30950827
Free PMC article.
Neutron structures of Leishmania mexicana triosephosphate isomerase in complex with reaction-intermediate mimics shed light on the proton-shuttling steps.
Kelpšas V, Caldararu O, Blakeley MP, Coquelle N, Wierenga RK, Ryde U, von Wachenfeldt C, Oksanen E.
Kelpšas V, et al.
IUCrJ. 2021 Jun 3;8(Pt 4):633-643. doi: 10.1107/S2052252521004619. eCollection 2021 Jul 1.
IUCrJ. 2021.
PMID: 34258011
Free PMC article.
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Evolving Escherichia coli Host Strains for Efficient Deuterium Labeling of Recombinant Proteins Using Sodium Pyruvate-d3.
Kelpšas V, Leung A, von Wachenfeldt C.
Kelpšas V, et al.
Int J Mol Sci. 2021 Sep 7;22(18):9678. doi: 10.3390/ijms22189678.
Int J Mol Sci. 2021.
PMID: 34575837
Free PMC article.
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Enhancing protein perdeuteration by experimental evolution of Escherichia coli K-12 for rapid growth in deuterium-based media.
Kelpšas V, von Wachenfeldt C.
Kelpšas V, et al.
Protein Sci. 2021 Dec;30(12):2457-2473. doi: 10.1002/pro.4206.
Protein Sci. 2021.
PMID: 34655136
Free PMC article.
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Strain improvement of Escherichia coli K-12 for recombinant production of deuterated proteins.
Kelpšas V, Wachenfeldt CV.
Kelpšas V, et al.
Sci Rep. 2019 Nov 27;9(1):17694. doi: 10.1038/s41598-019-54196-w.
Sci Rep. 2019.
PMID: 31776414
Free PMC article.
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