Complete refolding of bovine beta-lactoglobulin requires disulfide bond formation under strict conditions.
Hattori M, Hiramatsu K, Kurata T, Nishiura M, Takahashi K, Ametani A, Kaminogawa S.
Hattori M, et al. Among authors: kaminogawa s.
Biochim Biophys Acta. 2005 Sep 25;1752(2):154-65. doi: 10.1016/j.bbapap.2005.07.015.
Biochim Biophys Acta. 2005.
PMID: 16143573
beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retino …
beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed again …