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Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris.
Yurugi-Kobayashi T, Asada H, Shiroishi M, Shimamura T, Funamoto S, Katsuta N, Ito K, Sugawara T, Tokuda N, Tsujimoto H, Murata T, Nomura N, Haga K, Haga T, Iwata S, Kobayashi T. Yurugi-Kobayashi T, et al. Among authors: iwata s. Biochem Biophys Res Commun. 2009 Mar 6;380(2):271-6. doi: 10.1016/j.bbrc.2009.01.053. Epub 2009 Jan 22. Biochem Biophys Res Commun. 2009. PMID: 19167344
Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin.
Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Masuda K, Ishiguro M, Misaka T, Iwata S, Kobayashi T, Abe K. Ito K, et al. Among authors: iwata s. Biochim Biophys Acta. 2010 Sep;1800(9):986-92. doi: 10.1016/j.bbagen.2010.06.003. Epub 2010 Jun 11. Biochim Biophys Acta. 2010. PMID: 20542090 Free article.
Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein.
Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Misaka T, Iwata S, Kobayashi T, Abe K. Ito K, et al. Among authors: iwata s. Biotechnol Lett. 2011 Jan;33(1):103-7. doi: 10.1007/s10529-010-0399-1. Epub 2010 Oct 9. Biotechnol Lett. 2011. PMID: 20936326 Free article.
In vivo crystallography at X-ray free-electron lasers: the next generation of structural biology?
Gallat FX, Matsugaki N, Coussens NP, Yagi KJ, Boudes M, Higashi T, Tsuji D, Tatano Y, Suzuki M, Mizohata E, Tono K, Joti Y, Kameshima T, Park J, Song C, Hatsui T, Yabashi M, Nango E, Itoh K, Coulibaly F, Tobe S, Ramaswamy S, Stay B, Iwata S, Chavas LM. Gallat FX, et al. Among authors: iwata s. Philos Trans R Soc Lond B Biol Sci. 2014 Jul 17;369(1647):20130497. doi: 10.1098/rstb.2013.0497. Philos Trans R Soc Lond B Biol Sci. 2014. PMID: 24914164 Free PMC article.
Grease matrix as a versatile carrier of proteins for serial crystallography.
Sugahara M, Mizohata E, Nango E, Suzuki M, Tanaka T, Masuda T, Tanaka R, Shimamura T, Tanaka Y, Suno C, Ihara K, Pan D, Kakinouchi K, Sugiyama S, Murata M, Inoue T, Tono K, Song C, Park J, Kameshima T, Hatsui T, Joti Y, Yabashi M, Iwata S. Sugahara M, et al. Among authors: iwata s. Nat Methods. 2015 Jan;12(1):61-3. doi: 10.1038/nmeth.3172. Epub 2014 Nov 10. Nat Methods. 2015. PMID: 25384243 Free article.
Proteoliposome-based selection of a recombinant antibody fragment against the human M2 muscarinic acetylcholine receptor.
Suharni, Nomura Y, Arakawa T, Hino T, Abe H, Nakada-Nakura Y, Sato Y, Iwanari H, Shiroishi M, Asada H, Shimamura T, Murata T, Kobayashi T, Hamakubo T, Iwata S, Nomura N. Suharni, et al. Among authors: iwata s. Monoclon Antib Immunodiagn Immunother. 2014 Dec;33(6):378-85. doi: 10.1089/mab.2014.0041. Monoclon Antib Immunodiagn Immunother. 2014. PMID: 25545206 Free PMC article.
1,752 results