Factors affecting SFHR gene correction efficiency with single-stranded DNA fragment.
Tsuchiya H, Harashima H, Kamiya H.
Tsuchiya H, et al. Among authors: harashima h.
Biochem Biophys Res Commun. 2005 Nov 4;336(4):1194-200. doi: 10.1016/j.bbrc.2005.08.258.
Biochem Biophys Res Commun. 2005.
PMID: 16171787
A 606-nt single-stranded (ss) DNA fragment, prepared by restriction enzyme digestion of ss phagemid DNA, improves the gene correction efficiency by 12-fold as compared with a PCR fragment, which is the conventional type of fragment used in the small fragment homologous replacemen …
A 606-nt single-stranded (ss) DNA fragment, prepared by restriction enzyme digestion of ss phagemid DNA, improves the gene correction effici …