Goulding J - Search Results

1

Detection of genome-edited and endogenously expressed G protein-coupled receptors.

Soave M, Stoddart LA, White CW, Kilpatrick LE, Goulding J, Briddon SJ, Hill SJ. Soave M, et al. Among authors: goulding j. FEBS J. 2021 Apr;288(8):2585-2601. doi: 10.1111/febs.15729. Epub 2021 Feb 9. FEBS J. 2021. PMID: 33506623 Free PMC article. Review.

2

Application of BRET to monitor ligand binding to GPCRs.

Stoddart LA, Johnstone EKM, Wheal AJ, Goulding J, Robers MB, Machleidt T, Wood KV, Hill SJ, Pfleger KDG. Stoddart LA, et al. Among authors: goulding j. Nat Methods. 2015 Jul;12(7):661-663. doi: 10.1038/nmeth.3398. Epub 2015 Jun 1. Nat Methods. 2015. PMID: 26030448 Free PMC article.

3

Characterisation of endogenous A_2A and A_2B receptor-mediated cyclic AMP responses in HEK 293 cells using the GloSensor™ biosensor: Evidence for an allosteric mechanism of action for the A_2B-selective antagonist PSB 603.

Goulding J, May LT, Hill SJ. Goulding J, et al. Biochem Pharmacol. 2018 Jan;147:55-66. doi: 10.1016/j.bcp.2017.10.013. Epub 2017 Oct 26. Biochem Pharmacol. 2018. PMID: 29106905 Free PMC article.

4

Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy.

Soave M, Goulding J, Markus R, Hill SJ, Stoddart LA. Soave M, et al. Among authors: goulding j. Methods Mol Biol. 2020;2041:163-181. doi: 10.1007/978-1-4939-9717-6_12. Methods Mol Biol. 2020. PMID: 31646488

5

The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex.

Koo CZ, Harrison N, Noy PJ, Szyroka J, Matthews AL, Hsia HE, Müller SA, Tüshaus J, Goulding J, Willis K, Apicella C, Cragoe B, Davis E, Keles M, Malinova A, McFarlane TA, Morrison PR, Nguyen HTH, Sykes MC, Ahmed H, Di Maio A, Seipold L, Saftig P, Cull E, Pliotas C, Rubinstein E, Poulter NS, Briddon SJ, Holliday ND, Lichtenthaler SF, Tomlinson MG. Koo CZ, et al. Among authors: goulding j. J Biol Chem. 2020 Sep 4;295(36):12822-12839. doi: 10.1074/jbc.RA120.012601. Epub 2020 Feb 28. J Biol Chem. 2020. PMID: 32111735 Free PMC article.

6

Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles.

Grime RL, Goulding J, Uddin R, Stoddart LA, Hill SJ, Poyner DR, Briddon SJ, Wheatley M. Grime RL, et al. Among authors: goulding j. Nanoscale. 2020 Jun 4;12(21):11518-11525. doi: 10.1039/d0nr01060j. Nanoscale. 2020. PMID: 32428052

7

CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β₂AR in isogenic hPSC-derived cardiomyocytes.

Kondrashov A, Mohd Yusof NAN, Hasan A, Goulding J, Kodagoda T, Hoang DM, Vo NTN, Melarangi T, Dolatshad N, Gorelik J, Hill SJ, Harding SE, Denning C. Kondrashov A, et al. Among authors: goulding j. Mol Ther Methods Clin Dev. 2020 Oct 27;20:39-53. doi: 10.1016/j.omtm.2020.10.019. eCollection 2021 Mar 12. Mol Ther Methods Clin Dev. 2020. PMID: 33335946 Free PMC article.

8

The use of fluorescence correlation spectroscopy to monitor cell surface β2-adrenoceptors at low expression levels in human embryonic stem cell-derived cardiomyocytes and fibroblasts.

Goulding J, Kondrashov A, Mistry SJ, Melarangi T, Vo NTN, Hoang DM, White CW, Denning C, Briddon SJ, Hill SJ. Goulding J, et al. FASEB J. 2021 Apr;35(4):e21398. doi: 10.1096/fj.202002268R. FASEB J. 2021. PMID: 33710675

9

Subtype selective fluorescent ligands based on ICI 118,551 to study the human β2-adrenoceptor in CRISPR/Cas9 genome-edited HEK293T cells at low expression levels.

Goulding J, Mistry SJ, Soave M, Woolard J, Briddon SJ, White CW, Kellam B, Hill SJ. Goulding J, et al. Pharmacol Res Perspect. 2021 May;9(3):e00779. doi: 10.1002/prp2.779. Pharmacol Res Perspect. 2021. PMID: 34003582 Free PMC article.

10

Probing the binding of interleukin-23 to individual receptor components and the IL-23 heteromeric receptor complex in living cells using NanoBRET.

Lay CS, Bridges A, Goulding J, Briddon SJ, Soloviev Z, Craggs PD, Hill SJ. Lay CS, et al. Among authors: goulding j. Cell Chem Biol. 2022 Jan 20;29(1):19-29.e6. doi: 10.1016/j.chembiol.2021.05.002. Epub 2021 May 25. Cell Chem Biol. 2022. PMID: 34038748 Free PMC article.

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