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MPAPASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays.
Welsh JA, Killingsworth B, Kepley J, Traynor T, Cook S, Savage J, Marte J, Lee MJ, Maeng HM, Pleet ML, Magana S, Gorgens A, Maire CL, Lamszus K, Ricklefs FL, Merino MJ, Linehan WM, Greten T, Cooks T, Harris CC, Apolo A, Abdel-Mageed A, Ivanov AR, Trepel JB, Roth M, Tkach M, Milosavljevic A, Théry C, LeBlanc A, Berzofsky JA, Ruppin E, Aldape K, Camphausen K, Gulley JL, Ghiran I, Jacobson S, Jones JC. Welsh JA, et al. Among authors: gorgens a. Cell Rep Methods. 2022 Jan 24;2(1):100136. doi: 10.1016/j.crmeth.2021.100136. eCollection 2022 Jan 24. Cell Rep Methods. 2022. PMID: 35474866 Free PMC article.
Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures.
Wiklander OPB, Bostancioglu RB, Welsh JA, Zickler AM, Murke F, Corso G, Felldin U, Hagey DW, Evertsson B, Liang XM, Gustafsson MO, Mohammad DK, Wiek C, Hanenberg H, Bremer M, Gupta D, Björnstedt M, Giebel B, Nordin JZ, Jones JC, El Andaloussi S, Görgens A. Wiklander OPB, et al. Among authors: gorgens a. Front Immunol. 2018 Jun 13;9:1326. doi: 10.3389/fimmu.2018.01326. eCollection 2018. Front Immunol. 2018. PMID: 29951064 Free PMC article.
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.
Théry C, Witwer KW, Aikawa E, Alcaraz MJ, Anderson JD, Andriantsitohaina R, Antoniou A, Arab T, Archer F, Atkin-Smith GK, Ayre DC, Bach JM, Bachurski D, Baharvand H, Balaj L, Baldacchino S, Bauer NN, Baxter AA, Bebawy M, Beckham C, Bedina Zavec A, Benmoussa A, Berardi AC, Bergese P, Bielska E, Blenkiron C, Bobis-Wozowicz S, Boilard E, Boireau W, Bongiovanni A, Borràs FE, Bosch S, Boulanger CM, Breakefield X, Breglio AM, Brennan MÁ, Brigstock DR, Brisson A, Broekman ML, Bromberg JF, Bryl-Górecka P, Buch S, Buck AH, Burger D, Busatto S, Buschmann D, Bussolati B, Buzás EI, Byrd JB, Camussi G, Carter DR, Caruso S, Chamley LW, Chang YT, Chen C, Chen S, Cheng L, Chin AR, Clayton A, Clerici SP, Cocks A, Cocucci E, Coffey RJ, Cordeiro-da-Silva A, Couch Y, Coumans FA, Coyle B, Crescitelli R, Criado MF, D'Souza-Schorey C, Das S, Datta Chaudhuri A, de Candia P, De Santana EF, De Wever O, Del Portillo HA, Demaret T, Deville S, Devitt A, Dhondt B, Di Vizio D, Dieterich LC, Dolo V, Dominguez Rubio AP, Dominici M, Dourado MR, Driedonks TA, Duarte FV, Duncan HM, Eichenberger RM, Ekström K, El Andaloussi S, Elie-Caille C, Erdbrügger U, Falcón-Pérez JM, Fatima F, Fish JE, Flores-Bellver M, Försönit… See abstract for full author list ➔ Théry C, et al. Among authors: gorgens a. J Extracell Vesicles. 2018 Nov 23;7(1):1535750. doi: 10.1080/20013078.2018.1535750. eCollection 2018. J Extracell Vesicles. 2018. PMID: 30637094 Free PMC article.
Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material.
Görgens A, Bremer M, Ferrer-Tur R, Murke F, Tertel T, Horn PA, Thalmann S, Welsh JA, Probst C, Guerin C, Boulanger CM, Jones JC, Hanenberg H, Erdbrügger U, Lannigan J, Ricklefs FL, El-Andaloussi S, Giebel B. Görgens A, et al. J Extracell Vesicles. 2019 Mar 21;8(1):1587567. doi: 10.1080/20013078.2019.1587567. eCollection 2019. J Extracell Vesicles. 2019. PMID: 30949308 Free PMC article.
Imaging flow cytometry facilitates multiparametric characterization of extracellular vesicles in malignant brain tumours.
Ricklefs FL, Maire CL, Reimer R, Dührsen L, Kolbe K, Holz M, Schneider E, Rissiek A, Babayan A, Hille C, Pantel K, Krasemann S, Glatzel M, Heiland DH, Flitsch J, Martens T, Schmidt NO, Peine S, Breakefield XO, Lawler S, Chiocca EA, Fehse B, Giebel B, Görgens A, Westphal M, Lamszus K. Ricklefs FL, et al. Among authors: gorgens a. J Extracell Vesicles. 2019 Mar 21;8(1):1588555. doi: 10.1080/20013078.2019.1588555. eCollection 2019. J Extracell Vesicles. 2019. PMID: 30949309 Free PMC article.
MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments.
Welsh JA, Van Der Pol E, Arkesteijn GJA, Bremer M, Brisson A, Coumans F, Dignat-George F, Duggan E, Ghiran I, Giebel B, Görgens A, Hendrix A, Lacroix R, Lannigan J, Libregts SFWM, Lozano-Andrés E, Morales-Kastresana A, Robert S, De Rond L, Tertel T, Tigges J, De Wever O, Yan X, Nieuwland R, Wauben MHM, Nolan JP, Jones JC. Welsh JA, et al. Among authors: gorgens a. J Extracell Vesicles. 2020 Feb 3;9(1):1713526. doi: 10.1080/20013078.2020.1713526. eCollection 2020. J Extracell Vesicles. 2020. PMID: 32128070 Free PMC article.
Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging.
Gupta D, Liang X, Pavlova S, Wiklander OPB, Corso G, Zhao Y, Saher O, Bost J, Zickler AM, Piffko A, Maire CL, Ricklefs FL, Gustafsson O, Llorente VC, Gustafsson MO, Bostancioglu RB, Mamand DR, Hagey DW, Görgens A, Nordin JZ, El Andaloussi S. Gupta D, et al. Among authors: gorgens a. J Extracell Vesicles. 2020 Aug 21;9(1):1800222. doi: 10.1080/20013078.2020.1800222. J Extracell Vesicles. 2020. PMID: 32944187 Free PMC article.
Genome-wide methylation profiling of glioblastoma cell-derived extracellular vesicle DNA allows tumor classification.
Maire CL, Fuh MM, Kaulich K, Fita KD, Stevic I, Heiland DH, Welsh JA, Jones JC, Görgens A, Ricklefs T, Dührsen L, Sauvigny T, Joosse SA, Reifenberger G, Pantel K, Glatzel M, Miklosi AG, Felce JH, Caselli M, Pereno V, Reimer R, Schlüter H, Westphal M, Schüller U, Lamszus K, Ricklefs FL. Maire CL, et al. Among authors: gorgens a. Neuro Oncol. 2021 Jul 1;23(7):1087-1099. doi: 10.1093/neuonc/noab012. Neuro Oncol. 2021. PMID: 33508126 Free PMC article.
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