Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering).
Owen DM, Williamson D, Magenau A, Gaus K.
Owen DM, et al. Among authors: gaus k.
Methods Enzymol. 2012;504:221-35. doi: 10.1016/B978-0-12-391857-4.00011-2.
Methods Enzymol. 2012.
PMID: 22264537
Review.
Here, we describe a method for imaging membrane proteins using PALM, including live-cell PALM, to detect the molecular clustering of plasma membrane proteins using a statistical cluster-analysis method based on Ripley's K-function. While the method is applicable to a wide …
Here, we describe a method for imaging membrane proteins using PALM, including live-cell PALM, to detect the molecular clustering of plasma …