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The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. Götzke H, et al. Among authors: frey s. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. Nat Commun. 2019. PMID: 31562305 Free PMC article.
A new set of highly efficient, tag-cleaving proteases for purifying recombinant proteins.
Frey S, Görlich D. Frey S, et al. J Chromatogr A. 2014 Apr 11;1337:95-105. doi: 10.1016/j.chroma.2014.02.029. Epub 2014 Feb 19. J Chromatogr A. 2014. PMID: 24636565 Free article.
The most efficient protease, bdSENP1, is even more active and far more salt tolerant than its yeast ortholog scUlp1, allowing efficient tag removal also in high salt buffers containing, e.g. 1M NaCl. ssNEDP1 is distinguished by an exceptional salt tolerance, and a considerable to …
The most efficient protease, bdSENP1, is even more active and far more salt tolerant than its yeast ortholog scUlp1, allowing efficient tag …
898 results