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Immobilization of lipases by selective adsorption on hydrophobic supports.
Fernandez-Lafuente R, Armisén P, Sabuquillo P, Fernández-Lorente G, Guisán JM. Fernandez-Lafuente R, et al. Among authors: fernandez lorente g. Chem Phys Lipids. 1998 Jun;93(1-2):185-97. doi: 10.1016/s0009-3084(98)00042-5. Chem Phys Lipids. 1998. PMID: 9720258 Review.
Affinity chromatography of polyhistidine tagged enzymes. New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired multipoint adsorptions.
Mateo C, Fernandez-Lorente G, Pessela BC, Vian A, Carrascosa AV, Garcia JL, Fernandez-Lafuente R, Guisan JM. Mateo C, et al. Among authors: fernandez lorente g, fernandez lafuente r. J Chromatogr A. 2001 Apr 27;915(1-2):97-106. doi: 10.1016/s0021-9673(01)00626-4. J Chromatogr A. 2001. PMID: 11358266
Immobilization of Yarrowia lipolytica lipase--a comparison of stability of physical adsorption and covalent attachment techniques.
Cunha AG, Fernández-Lorente G, Bevilaqua JV, Destain J, Paiva LM, Freire DM, Fernández-Lafuente R, Guisán JM. Cunha AG, et al. Among authors: fernandez lorente g, fernandez lafuente r. Appl Biochem Biotechnol. 2008 Mar;146(1-3):49-56. doi: 10.1007/s12010-007-8073-3. Epub 2007 Nov 9. Appl Biochem Biotechnol. 2008. PMID: 18421586
Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose.
Fernandez-Lorente G, Godoy CA, Mendes AA, Lopez-Gallego F, Grazu V, de Las Rivas B, Palomo JM, Hermoso J, Fernandez-Lafuente R, Guisan JM. Fernandez-Lorente G, et al. Among authors: fernandez lafuente r. Biomacromolecules. 2008 Sep;9(9):2553-61. doi: 10.1021/bm800609g. Epub 2008 Aug 15. Biomacromolecules. 2008. PMID: 18702542
Separation and immobilization of lipase from Penicillium simplicissimum by selective adsorption on hydrophobic supports.
Cunha AG, Fernández-Lorente G, Gutarra ML, Bevilaqua JV, Almeida RV, Paiva LM, Fernández-Lafuente R, Guisán JM, Freire DM. Cunha AG, et al. Among authors: fernandez lorente g, fernandez lafuente r. Appl Biochem Biotechnol. 2009 May;156(1-3):133-45. doi: 10.1007/s12010-008-8425-7. Epub 2008 Nov 28. Appl Biochem Biotechnol. 2009. PMID: 19037600
Protein hydrolysis by immobilized and stabilized trypsin.
Marques D, Pessela BC, Betancor L, Monti R, Carrascosa AV, Rocha-Martin J, Guisán JM, Fernandez-Lorente G. Marques D, et al. Among authors: fernandez lorente g. Biotechnol Prog. 2011 May-Jun;27(3):677-83. doi: 10.1002/btpr.600. Epub 2011 Apr 20. Biotechnol Prog. 2011. PMID: 21509952
71 results