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Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
STAR Protoc. 2023 Mar 17;4(1):101974. doi: 10.1016/j.xpro.2022.101974. Epub 2023 Jan 11.
STAR Protoc. 2023.
PMID: 36633947
Free PMC article.
Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells.
Schumann B, Malaker SA, Wisnovsky SP, Debets MF, Agbay AJ, Fernandez D, Wagner LJS, Lin L, Li Z, Choi J, Fox DM, Peh J, Gray MA, Pedram K, Kohler JJ, Mrksich M, Bertozzi CR.
Schumann B, et al.
Mol Cell. 2020 Jun 4;78(5):824-834.e15. doi: 10.1016/j.molcel.2020.03.030. Epub 2020 Apr 22.
Mol Cell. 2020.
PMID: 32325029
Free PMC article.
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