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G13A substitution affects the biochemical and physical properties of the elongation factor 1 alpha. A reduced intrinsic GTPase activity is partially restored by kirromycin.
Masullo M, Cantiello P, de Paola B, Catanzano F, Arcari P, Bocchini V. Masullo M, et al. Among authors: bocchini v. Biochemistry. 2002 Jan 15;41(2):628-33. doi: 10.1021/bi015598h. Biochemistry. 2002. PMID: 11781103
., Adinolfi, B. S., Amatruda, M. R., Raimo, G., Masullo, M., and Bocchini, V. (1994) Eur. J. Biochem. 262, 600-605]. In this work, we found that, compared to the wild-type factor (SsEF-1 alpha wt), G13ASsEF-1 alpha shows (i) a reduced rate of [(3)H]Phe polymerizatio …
., Adinolfi, B. S., Amatruda, M. R., Raimo, G., Masullo, M., and Bocchini, V. (1994) Eur. J. Biochem. 262, 600-605]. In this w …
The A26G replacement in the consensus sequence A-X-X-X-X-G-K-[T,S] of the guanine nucleotide binding site activates the intrinsic GTPase of the elongation factor 2 from the archaeon Sulfolobus solfataricus.
De Vendittis E, Adinolfi BS, Amatruda MR, Raimo G, Masullo M, Bocchini V. De Vendittis E, et al. Among authors: bocchini v. Eur J Biochem. 1999 Jun;262(2):600-5. doi: 10.1046/j.1432-1327.1999.00428.x. Eur J Biochem. 1999. PMID: 10336648 Free article.
In contrast, the A26G substitution enhanced the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol [Raimo, G., Masullo, M., Scarano, G., & Bocchini, V. (1997) Biochimie 78, 832-837]. Surprisingly, A26GSsEF-2 was able to hydrolyse GT …
In contrast, the A26G substitution enhanced the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol [Raimo, G., …
Salts induce structural changes in elongation factor 1alpha from the hyperthermophilic archaeon Sulfolobus solfataricus: a Fourier transform infrared spectroscopic study.
Tanfani F, Scirè A, Masullo M, Raimo G, Bertoli E, Bocchini V. Tanfani F, et al. Among authors: bocchini v. Biochemistry. 2001 Nov 6;40(44):13143-8. doi: 10.1021/bi0101291. Biochemistry. 2001. PMID: 11683622
Elongation factor 1alpha from the hyperthermophilic archaeon Sulfolobus solfataricus (SsEF-1alpha) carries the aminoacyl tRNA to the ribosome; it binds GDP or GTP, and it is also endowed with an intrinsic GTPase activity that is triggered in vitro by NaCl at molar concentrations …
Elongation factor 1alpha from the hyperthermophilic archaeon Sulfolobus solfataricus (SsEF-1alpha) carries the aminoacyl tRNA to the ribosom …
121 results