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Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in Escherichia coli, Purification, and Characterization.
Cserjan-Puschmann M, Lingg N, Engele P, Kröß C, Loibl J, Fischer A, Bacher F, Frank AC, Öhlknecht C, Brocard C, Oostenbrink C, Berkemeyer M, Schneider R, Striedner G, Jungbauer A. Cserjan-Puschmann M, et al. Among authors: berkemeyer m. Biomolecules. 2020 Nov 24;10(12):1592. doi: 10.3390/biom10121592. Biomolecules. 2020. PMID: 33255244 Free PMC article.
CASPON platform technology: Ultrafast circularly permuted caspase-2 cleaves tagged fusion proteins before all 20 natural amino acids at the N-terminus.
Lingg N, Kröß C, Engele P, Öhlknecht C, Köppl C, Fischer A, Lier B, Loibl J, Sprenger B, Liu J, Scheidl P, Berkemeyer M, Buchinger W, Brocard C, Striedner G, Oostenbrink C, Schneider R, Jungbauer A, Cserjan-Puschmann M. Lingg N, et al. Among authors: berkemeyer m. N Biotechnol. 2022 Nov 25;71:37-46. doi: 10.1016/j.nbt.2022.07.002. Epub 2022 Aug 1. N Biotechnol. 2022. PMID: 35926774 Free article.
Refolding in the modern biopharmaceutical industry.
Buscajoni L, Martinetz MC, Berkemeyer M, Brocard C. Buscajoni L, et al. Among authors: berkemeyer m. Biotechnol Adv. 2022 Dec;61:108050. doi: 10.1016/j.biotechadv.2022.108050. Epub 2022 Oct 15. Biotechnol Adv. 2022. PMID: 36252795 Free article. Review.