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Modulation of the microenvironment surrounding the active site of penicillin G acylase immobilized on acrylic carriers improves the enzymatic synthesis of cephalosporins.
Molecules. 2013 Nov 20;18(11):14349-65. doi: 10.3390/molecules181114349.
Molecules. 2013.
PMID: 24264137
Free PMC article.
Characterization and study of the orientation of immobilized enzymes by tryptic digestion and HPLC-MS: design of an efficient catalyst for the synthesis of cephalosporins.
Temporini C, Bonomi P, Serra I, Tagliani A, Bavaro T, Ubiali D, Massolini G, Terreni M.
Temporini C, et al. Among authors: tagliani a.
Biomacromolecules. 2010 Jun 14;11(6):1623-32. doi: 10.1021/bm100259a.
Biomacromolecules. 2010.
PMID: 20462204
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Immobilization of the acylase from Escherichia coli on glyoxyl-agarose gives efficient catalyst for the synthesis of cephalosporins.
Estruch I, Tagliani AR, Guisán JM, Fernández-Lafuente R, Alcántara AR, Toma L, Terreni M.
Estruch I, et al. Among authors: tagliani ar.
Enzyme Microb Technol. 2008 Jan;42(2):121-9. doi: 10.1016/j.enzmictec.2007.08.013. Epub 2007 Sep 6.
Enzyme Microb Technol. 2008.
PMID: 22578861
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