Directed evolution of aniline dioxygenase for enhanced bioremediation of aromatic amines.
Ang EL, Obbard JP, Zhao H.
Ang EL, et al.
Appl Microbiol Biotechnol. 2009 Jan;81(6):1063-70. doi: 10.1007/s00253-008-1710-0. Epub 2008 Sep 24.
Appl Microbiol Biotechnol. 2009.
PMID: 18813921
Previously, the mutation V205A was found to widen the substrate specificity of AtdA to accept 2-isopropylaniline (2IPA) for which the wild-type enzyme has no activity (Ang EL, Obbard JP, Zhao HM, FEBS J, 274:928-939, 2007). Using mutant V205A as the parent and apply …
Previously, the mutation V205A was found to widen the substrate specificity of AtdA to accept 2-isopropylaniline (2IPA) for which the wild-t …