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Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid.
Jiang H, Xing Z, Liu X, Chai Q, Xin Z, Zhu C, Lin R, Deng X, Cui D, Gao H, Ma D. Jiang H, et al. BMC Infect Dis. 2022 Apr 1;22(1):326. doi: 10.1186/s12879-022-07272-y. BMC Infect Dis. 2022. PMID: 35365081 Free PMC article.
Microbial DNA and total RNA increased microbial deposition by depleting the host DNA. Methods total NA and total RNA can detect gram-positive, gram-negative, RNA and DNA pathogens. We applied mNGS, including total NA and NEB library generation, to CSF samples from f …
Microbial DNA and total RNA increased microbial deposition by depleting the host DNA. Methods total NA and total RNA can detect gram- …
Development of a molecular K+ probe for colorimetric/fluorescent/photoacoustic detection of K.
Ning J, Lin X, Su F, Sun A, Liu H, Luo J, Wang L, Tian Y. Ning J, et al. Anal Bioanal Chem. 2020 Oct;412(25):6947-6957. doi: 10.1007/s00216-020-02826-y. Epub 2020 Jul 25. Anal Bioanal Chem. 2020. PMID: 32712812
NK2 shows good response to K(+) with obvious color changes, fluorescence enhancements (about threefold), and photoacoustic intensity changes. The existence of other metal ions (including Na(+), Mg(2+), Ca(2+), Fe(2+)) and pH changes (6.5-9.0) have no obvious influence on K …
NK2 shows good response to K(+) with obvious color changes, fluorescence enhancements (about threefold), and photoacoustic intensity changes …