Transgene-free CRISPR/Cas9-mediated gene editing through protoplast-to-plant regeneration enhances active compounds in Salvia miltiorrhiza

Affiliations

• ¹ Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
• ² Institute of Tropical Plant Sciences and Microbiology, National Cheng Kung University, Tainan, Taiwan.
• ³ Biotechnology Research Center in Southern Taiwan, Academia Sinica, Tainan, Taiwan.
• ⁴ Bioinnovation Center, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
• ⁵ Department of Pathology, Hualien Tzu Chi Hospital and Tzu Chi University, Hualien, Taiwan.
• ⁶ Department of Neurosurgery, Hualien Tzu Chi Hospital, Hualien, Taiwan.