Recombinant 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase (ErtC) derived from Burkholderia sp. HME13 was purified to homogeneity. Here, ErtC's kinetic parameters, optimum reaction temperature and pH, and stability at varying temperatures and pH and the effects of various additives on ErtC activity were determined. Real-time polymerase chain reaction and enzyme assays suggested that ergothioneine induced the expression of ertC.
Keywords: Burkholderia; 3-(2,5-dioxoimidazolidin-4-yl) propionic acid; 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase; DUF917 protein family; ergothioneine.
© The Author(s) 2023. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.