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Rapid and efficient detection methods of pathogenic swine enteric coronaviruses.
Fu Y, Li B, Liu G. Fu Y, et al. Appl Microbiol Biotechnol. 2020 Jul;104(14):6091-6100. doi: 10.1007/s00253-020-10645-5. Epub 2020 May 19. Appl Microbiol Biotechnol. 2020. PMID: 32430534 Free PMC article. Review.
This paper reviews various PCR-based methods used for the rapid and efficient detection of these pathogenic CoVs in swine intestines. KEY POINTS: 1. Swine enteric coronaviruses (CoVs) emerged and reemerged in past years. 2. ...
This paper reviews various PCR-based methods used for the rapid and efficient detection of these pathogenic CoVs in swine intestines. KEY …
A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses.
Huang X, Chen J, Yao G, Guo Q, Wang J, Liu G. Huang X, et al. Appl Microbiol Biotechnol. 2019 Jun;103(12):4943-4952. doi: 10.1007/s00253-019-09835-7. Epub 2019 Apr 26. Appl Microbiol Biotechnol. 2019. PMID: 31025076 Free PMC article.
The results showed that the limit of detection can reach as low as 10 copies in singular real-time RT-qPCR assays and 100 copies in multiplex real-time RT-qPCR assay, with all correlation coefficients (R(2)) at above 0.99, and the amplification efficiency at between 90 and …
The results showed that the limit of detection can reach as low as 10 copies in singular real-time RT-qPCR assays and 100 copies in multiple …