Assignment of human sprouty 4 gene to chromosome segment 5q32 approximately 33 and analysis of its pattern of expression

Hua Liu; Jin-Zhong Chen; Shao-Hua Gu; Jian-Liang Dai; En-Pang Zhao; Lu Huang; Wang-Xiang Xu; Yi Xie; Yu-Min Mao

doi:10.1007/BF02715877

Assignment of human sprouty 4 gene to chromosome segment 5q32 approximately 33 and analysis of its pattern of expression

J Genet. 2003 Apr-Aug;82(1-2):23-6. doi: 10.1007/BF02715877.

Authors

Hua Liu¹, Jin-Zhong Chen, Shao-Hua Gu, Jian-Liang Dai, En-Pang Zhao, Lu Huang, Wang-Xiang Xu, Yi Xie, Yu-Min Mao

Affiliation

¹ State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China. ymmao@fudan.edu.cn

PMID: 14631098
DOI: 10.1007/BF02715877

Abstract

The human sprouty 4 (SPRY4) gene was localized to chromosome band 5q32 approximately 33 by screening the Stanford radiation hybrid G3 panel using a SPRY4-specific primer pair for PCR. Northern blot analysis revealed two different mRNAs (5 kb and 2 kb) in liver, skeletal muscle, heart, lung, kidney, spleen, placenta and small intestine. Reverse transcriptase-PCR analysis showed that SPRY4 was expressed in all tested tissues to different levels.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Blotting, Northern
Chromosome Mapping*
Chromosomes, Human, Pair 5*
DNA Primers / chemistry
Humans
Intracellular Signaling Peptides and Proteins
Nerve Tissue Proteins
Proteins / genetics*
Proteins / metabolism
RNA, Messenger / metabolism
Radiation Hybrid Mapping
Reverse Transcriptase Polymerase Chain Reaction
Tissue Distribution

Substances

DNA Primers
Intracellular Signaling Peptides and Proteins
Nerve Tissue Proteins
Proteins
RNA, Messenger
SPRY4 protein, human