Ultrasensitive detection of microRNAs using catalytic hairpin assembly coupled with enzymatic repairing amplification

Chong-Hua Zhang; Ying Tang; Ying-Ying Sheng; Hui Wang; Zhan Wu; Jian-Hui Jiang

doi:10.1039/c6cc07116c

Ultrasensitive detection of microRNAs using catalytic hairpin assembly coupled with enzymatic repairing amplification

Chem Commun (Camb). 2016 Nov 15;52(93):13584-13587. doi: 10.1039/c6cc07116c.

Authors

Chong-Hua Zhang¹, Ying Tang¹, Ying-Ying Sheng¹, Hui Wang¹, Zhan Wu¹, Jian-Hui Jiang¹

Affiliation

¹ State Key Laboratory of Chemo-Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China. jianhuijiang@hnu.edu.cn wuzhan1002@gmail.com.

PMID: 27805185
DOI: 10.1039/c6cc07116c

Abstract

A novel isothermal nucleic acid amplification technology is developed by coupling catalytic hairpin assembly with enzymatic repairing amplification as a highly sensitive and selective platform for miRNA detection.