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Methodological considerations for measuring biofluid-based microRNA biomarkers.
Chorley BN, Atabakhsh E, Doran G, Gautier JC, Ellinger-Ziegelbauer H, Jackson D, Sharapova T, Yuen PST, Church RJ, Couttet P, Froetschl R, McDuffie J, Martinez V, Pande P, Peel L, Rafferty C, Simutis FJ, Harrill AH. Chorley BN, et al. Crit Rev Toxicol. 2021 Mar;51(3):264-282. doi: 10.1080/10408444.2021.1907530. Epub 2021 May 26. Crit Rev Toxicol. 2021. PMID: 34038674 Free PMC article.
Absolute Measurement of Cardiac Injury-Induced microRNAs in Biofluids across Multiple Test Sites.
Thompson KL, Boitier E, Chen T, Couttet P, Ellinger-Ziegelbauer H, Goetschy M, Guillemain G, Kanki M, Kelsall J, Mariet C, de La Moureyre-Spire C, Mouritzen P, Nassirpour R, O'Lone R, Pine PS, Rosenzweig BA, Sharapova T, Smith A, Uchiyama H, Yan J, Yuen PS, Wolfinger R. Thompson KL, et al. Toxicol Sci. 2016 Nov;154(1):115-125. doi: 10.1093/toxsci/kfw143. Epub 2016 Sep 7. Toxicol Sci. 2016. PMID: 27605421
Two approaches for estimating the lower limit of quantitation (LLOQ) of microRNA levels assayed as exploratory biomarkers by RT-qPCR.
Wolfinger RD, Beedanagari S, Boitier E, Chen T, Couttet P, Ellinger-Ziegelbauer H, Guillemain G, Mariet C, Mouritzen P, O'Lone R, Pine PS, Sharapova T, Yan J, Yuen PS, Thompson KL. Wolfinger RD, et al. BMC Biotechnol. 2018 Feb 2;18(1):6. doi: 10.1186/s12896-018-0415-4. BMC Biotechnol. 2018. PMID: 29391006 Free PMC article.