Pot experiment with tobacco shows that the amount of total, solube, exchangeable and organic Se in rhizospheric soil appeared depletory, while that of total, adsorbed and organic S appeared enriched. The influence of added S on Se depletion depended on the level of Se application, and that of added Se on S enrichment depended on the rate of S applicagtion. In the absence of added Se, the addition of S decreased the depletion of several Se forms; while in its presence, the reverse result was obtained. The addition of Se increased the enrichment of total S when the S was not added. With S application, the added Se reduced the enrichment of total S. At early stage, teh Se content of tobacco was mainly influenced by the soluble Se in rhizospheric soil, while the accumulated amount of Se in tobacco was also influenced by exchangeable, organic and acid soluble Se. The S content in the aboveground part of tobacco at its early stage was mainly influenced by soluble and organic S in the rhizosphere.