Use of hexon as an antigen for the production of monoclonal antibodies capable of detecting multiple adenovirus types

Tatyana A Timoshicheva; Yana A Zabrodskaya; Edward Ramsay; Irina V Amosova

doi:10.1016/j.biologicals.2019.01.006

Use of hexon as an antigen for the production of monoclonal antibodies capable of detecting multiple adenovirus types

Biologicals. 2019 Mar:58:44-49. doi: 10.1016/j.biologicals.2019.01.006. Epub 2019 Jan 17.

Authors

Tatyana A Timoshicheva¹, Yana A Zabrodskaya², Edward Ramsay¹, Irina V Amosova¹

Affiliations

¹ Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, prof. Popov St. 15/17, St. Petersburg, Russia.
² Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, prof. Popov St. 15/17, St. Petersburg, Russia; Petersburg Nuclear Physics Institute named after B. P. Konstantinov of National Research Center "Kurchatov Institute", 188300, mkr. Orlova roshcha 1, Gatchina, Russia. Electronic address: yana@zabrodskaya.net.

PMID: 30661900
DOI: 10.1016/j.biologicals.2019.01.006

Abstract

In this work, monoclonal antibodies to the adenovirus protein hexon were produced, and their biological and diagnostic properties were characterized. The specific activities of the new monoclonal antibodies, with respect to various adenovirus types, were studied by enzyme-linked immunosorbent assay, indirect immunofluorescence, and western blot analysis. The data demonstrate the potential of the monoclonal antibodies developed, namely 4B7 and 6B12, for use in the development of modern diagnostic assays.

Keywords: Adenovirus; Hexon; Monoclonal antibodies.

MeSH terms

A549 Cells
Adenoviridae / immunology*
Animals
Antibodies, Monoclonal, Murine-Derived / immunology*
Antibodies, Viral / immunology*
Antigens, Viral / immunology*
Capsid Proteins / immunology*
Humans
Mice
Mice, Inbred BALB C

Substances

Antibodies, Monoclonal, Murine-Derived
Antibodies, Viral
Antigens, Viral
Capsid Proteins
hexon capsid protein, Adenovirus