Objective: To measure the effect of the eukaryotic expression vector of siRNA specific for hnRNP B1 on the expression of hnRNP Bx in lung cancer tissues in vivo.
Methods: The eukaryotic expression vector of siRNA specific for hnRNP B1 was inoculated to nude mice with lung cancers. The mRNA and protein expressions of hnRNP B1 in the lung cancer tissues of the nude mice were detected by RT-PCR and Western blot. The optimal conditions for the expressions were identified.
Results: The eukaryotic expression vector of siRNA specific for hnRNP B1 inhibited the mRNA and protein expressions at 40 days after the inoculation. However, the inhibitive effect disappeared at 60 days after the inoculation.
Conclusion: The expression of hnRNP B1 gene in A549 cells can be inhibited by hnRNP Br-specific siRNA expression vectors.