[Application of Multiplex PCR for the Screening Di(b-),Co(a-) Rare Blood Group in Chinese Xinjiang Minorities]

Objective: To investigate the distribution of Colton and Diego rare blood group antigens of blood donors in Chinese Xinjiang minorities.

Methods: A multiplex PCR was applied to screen for alleles antigens Di^a and Co^b in 1020 randomly selected healthy donors of Chinese Xinjiang minorities by using each 5 samples mixed detection method. The samples in the positive pools were further tested individually. Furthermore, the positive samples, including Di^(a+b-)/Di^(a+b+) and Co^(a-b+)/ Co^(a+b+) genotypes were tested via 2 PCR-SSP assays for high frequency allele Di^b and Co^a to get the rare genotypes Di^(b-), Co^(a-).

Results: Among 1020 samples 12 cases with Co^b+ allele, 45 cases with Di^(a+b+) and 1 case with Di^(a+b-) were identified.

Conclusion: The frequencies of Di^a and Co^b alleles are 2.30% and 0.59%, respectively. The information of rare blood donors obtained from the screening can provide a reference for matched blood transfusion, and further enrich the National Rare Blood Bank of China.