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Page 1
TraA, TraC and TraD autorepress two divergent quorum-regulated promoters near the transfer origin of the Ti plasmid of Agrobacterium tumefaciens.
Cho H, Winans SC. Cho H, et al. Mol Microbiol. 2007 Mar;63(6):1769-82. doi: 10.1111/j.1365-2958.2007.05624.x. Mol Microbiol. 2007. PMID: 17367394 Free article.
Likewise, Ti plasmid conjugation required TraA, TraF and TraG, and occurred at reduced levels in the absence of TraC or TraD. TraA preferentially acted in cis in repressing the traA and traC promoters and in the processing of oriT, which explain …
Likewise, Ti plasmid conjugation required TraA, TraF and TraG, and occurred at reduced levels in the absence of TraC or TraD. …
Cloning, E. coli overexpression, purification and binding properties of TraA and TraC, two proteins involved in the pheromone-dependent conjugation process in enterococci.
Alfieri B, Folloni S, Elviri L, Gobbo M, Berni R, Folli C. Alfieri B, et al. Protein Expr Purif. 2008 Aug;60(2):198-204. doi: 10.1016/j.pep.2008.03.009. Epub 2008 Mar 25. Protein Expr Purif. 2008. PMID: 18468916
We have isolated the sequences encoding TraA and TraC from the plasmid pPD1 and cloned them in suitable expression vectors. ...Overall these results are consistent with the heterologously expressed TraC and TraA being functionally active....
We have isolated the sequences encoding TraA and TraC from the plasmid pPD1 and cloned them in suitable expression vectors. .. …
Molecular mechanism of peptide-specific pheromone signaling in Enterococcus faecalis: functions of pheromone receptor TraA and pheromone-binding protein TraC encoded by plasmid pPD1.
Nakayama J, Takanami Y, Horii T, Sakuda S, Suzuki A. Nakayama J, et al. J Bacteriol. 1998 Feb;180(3):449-56. doi: 10.1128/JB.180.3.449-456.1998. J Bacteriol. 1998. PMID: 9457843 Free PMC article.
A recombinant TraA exhibited a dissociation constant of 0.49 +/- 0.08 nM against [3H]cPD1. iPD1 competitively inhibited [3H]cPD1 binding to TraA, whereas pheromones and inhibitors relating to other plasmid systems did not. These results show that TraA is a sp …
A recombinant TraA exhibited a dissociation constant of 0.49 +/- 0.08 nM against [3H]cPD1. iPD1 competitively inhibited [3H]cPD1 bind …
Enterococcus faecalis sex pheromone plasmid pAM373: analyses of TraA and evidence for its interaction with RpoB.
Ozawa Y, De Boever EH, Clewell DB. Ozawa Y, et al. Plasmid. 2005 Jul;54(1):57-69. doi: 10.1016/j.plasmid.2004.12.003. Epub 2005 Feb 1. Plasmid. 2005. PMID: 15907539
In addition the promoter of traA, designated P(a), was shown to be located about 600bp upstream of the translational start site, as clones containing traA required this site to complement traA mutants in trans. Transcription from P(a) also gave rise to a shor …
In addition the promoter of traA, designated P(a), was shown to be located about 600bp upstream of the translational start site, as c …
Promiscuous DNA transfer system of Agrobacterium tumefaciens: role of the virB operon in sex pilus assembly and synthesis.
Kado CI. Kado CI. Mol Microbiol. 1994 Apr;12(1):17-22. doi: 10.1111/j.1365-2958.1994.tb00990.x. Mol Microbiol. 1994. PMID: 7914664 Review.
The proteins encoded by the PilW operon of IncW plasmid R388 share strong similarities (average similarity = 50.8%) with VirB proteins. Similarly, the TraA, TraL and TraC proteins of IncF plasmid F have similarities to VirB2, VirB3 and VirB4 respectively (average si …
The proteins encoded by the PilW operon of IncW plasmid R388 share strong similarities (average similarity = 50.8%) with VirB proteins. Simi …
Cloning and characterization of a region of Enterococcus faecalis plasmid pPD1 encoding pheromone inhibitor (ipd), pheromone sensitivity (traC), and pheromone shutdown (traB) genes.
Nakayama J, Yoshida K, Kobayashi H, Isogai A, Clewell DB, Suzuki A. Nakayama J, et al. J Bacteriol. 1995 Oct;177(19):5567-73. doi: 10.1128/jb.177.19.5567-5573.1995. J Bacteriol. 1995. PMID: 7559344 Free PMC article.
A 5.4-kb EcoRV-HincII segment of pPD1, which expressed iPD1 in Escherichia coli, was sequenced and found to be organized as traC-traB-traA-ipd; each open reading frame is analogous to that found in other pheromone plasmids, pAD1 and pCF10, and thus is designated in …
A 5.4-kb EcoRV-HincII segment of pPD1, which expressed iPD1 in Escherichia coli, was sequenced and found to be organized as traC-traB …
Modular Lipoprotein Toxins Transferred by Outer Membrane Exchange Target Discrete Cell Entry Pathways.
Vassallo CN, Sah GP, Weltzer ML, Wall D. Vassallo CN, et al. mBio. 2021 Oct 26;12(5):e0238821. doi: 10.1128/mBio.02388-21. Epub 2021 Sep 14. mBio. 2021. PMID: 34517761 Free PMC article.
Importantly, a mutant that confers resistance to all six SitA families was discovered. This gene was named traC and plays an accessory role with traAB in OME. This work thus provides insight into the mechanism of kin discrimination in myxobacteria and provides working mode …
Importantly, a mutant that confers resistance to all six SitA families was discovered. This gene was named traC and plays an accessor …
The tra region of the nopaline-type Ti plasmid is a chimera with elements related to the transfer systems of RSF1010, RP4, and F.
Farrand SK, Hwang I, Cook DM. Farrand SK, et al. J Bacteriol. 1996 Jul;178(14):4233-47. doi: 10.1128/jb.178.14.4233-4247.1996. J Bacteriol. 1996. PMID: 8763953 Free PMC article.
The region contains six identifiable genes organized as two units divergently transcribable from a 258-bp inter-genic region that contains the oriT site. One unit encodes traA, traF, and traB, while the second encodes traC, traD, and traG. ...The 127-kDa protein exp …
The region contains six identifiable genes organized as two units divergently transcribable from a 258-bp inter-genic region that contains t …
Transcriptional regulation of pWW0 transfer genes in Pseudomonas putida KT2440.
Lambertsen LM, Molin S, Kroer N, Thomas CM. Lambertsen LM, et al. Plasmid. 2004 Nov;52(3):169-81. doi: 10.1016/j.plasmid.2004.06.005. Plasmid. 2004. PMID: 15518874
In this study we show that the IncP-9 transfer genes are transcribed from at least three promoter regions. The promoters for traA and traD act divergently from the region found to encode the origin of transfer, oriT. These promoters regulate expression of traA, B, a …
In this study we show that the IncP-9 transfer genes are transcribed from at least three promoter regions. The promoters for traA and …
Analysis of the transfer region of the Streptomyces plasmid SCP2.
Brolle DF, Pape H, Hopwood DA, Kieser T. Brolle DF, et al. Mol Microbiol. 1993 Oct;10(1):157-70. doi: 10.1111/j.1365-2958.1993.tb00912.x. Mol Microbiol. 1993. PMID: 7968512
Mutant plasmids differing in their transfer frequencies, chromosome mobilization abilities, pock formation, and complementation properties were isolated. The mutations defined five transfer-related genes, traA, traB, traC, traD and spd, clustered in a region of 9 kb …
Mutant plasmids differing in their transfer frequencies, chromosome mobilization abilities, pock formation, and complementation properties w …
27 results