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2008 1908
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67,858 results

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Page 1
RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues.
Wang F, Flanagan J, Su N, Wang LC, Bui S, Nielson A, Wu X, Vo HT, Ma XJ, Luo Y. Wang F, et al. J Mol Diagn. 2012 Jan;14(1):22-9. doi: 10.1016/j.jmoldx.2011.08.002. J Mol Diagn. 2012. PMID: 22166544 Free PMC article.
Immunohistochemistry and DNA in situ hybridization (ISH) are widely used in clinical settings to assess protein and DNA biomarkers, respectively, but clinical use of in situ RNA analysis is rare. ...RNAscope is compatible with routine formalin-fixed, paraffin …
Immunohistochemistry and DNA in situ hybridization (ISH) are widely used in clinical settings to assess protein and DNA biomarkers, r …
Global in situ profiling of RNA-RNA spatial interactions with RIC-seq.
Cao C, Cai Z, Ye R, Su R, Hu N, Zhao H, Xue Y. Cao C, et al. Nat Protoc. 2021 Jun;16(6):2916-2946. doi: 10.1038/s41596-021-00524-2. Epub 2021 May 21. Nat Protoc. 2021. PMID: 34021296
RNA in situ conformation sequencing (RIC-seq) is a technique for capturing protein-mediated RNA-RNA proximal interactions globally in living cells at single-base resolution. Cells are first treated with formaldehyde to fix all the protein-mediated R …
RNA in situ conformation sequencing (RIC-seq) is a technique for capturing protein-mediated RNA-RNA proximal int …
In Situ Amplification-Based Imaging of RNA in Living Cells.
Qing Z, Xu J, Hu J, Zheng J, He L, Zou Z, Yang S, Tan W, Yang R. Qing Z, et al. Angew Chem Int Ed Engl. 2019 Aug 19;58(34):11574-11585. doi: 10.1002/anie.201812449. Epub 2019 May 14. Angew Chem Int Ed Engl. 2019. PMID: 30707484 Review.
Owing to its important physiological functions, especially as molecular biomarkers of diseases, RNA is an important focus of biomedicine and biochemical sensing. ...However, conventional amplification methods for RNA analysis depend on the use of enzymes, fixation o …
Owing to its important physiological functions, especially as molecular biomarkers of diseases, RNA is an important focus of biomedic …
[RNA in situ hybridization: technology, potential, and fields of application].
Schipper C, Zielinski D. Schipper C, et al. Pathologe. 2020 Nov;41(6):563-573. doi: 10.1007/s00292-020-00839-z. Pathologe. 2020. PMID: 32997158 Review. German.
Significant improvements in the technology of RNA in situ hybridization (RNA-ISH) in the past five decades have opened up novel fields of its application as a valuable and an attractive adjunct to the portfolio of pathologist's daily routine diagnostic practi …
Significant improvements in the technology of RNA in situ hybridization (RNA-ISH) in the past five decades have opened …
Nanoscale imaging of RNA with expansion microscopy.
Chen F, Wassie AT, Cote AJ, Sinha A, Alon S, Asano S, Daugharthy ER, Chang JB, Marblestone A, Church GM, Raj A, Boyden ES. Chen F, et al. Nat Methods. 2016 Aug;13(8):679-84. doi: 10.1038/nmeth.3899. Epub 2016 Jul 4. Nat Methods. 2016. PMID: 27376770 Free PMC article.
Here, we present a strategy for expansion microscopy of RNA. We developed a small-molecule linker that enables RNA to be covalently attached to a swellable polyelectrolyte gel synthesized throughout a biological specimen. Then, postexpansion, fluorescent in situ …
Here, we present a strategy for expansion microscopy of RNA. We developed a small-molecule linker that enables RNA to be coval …
Robust and sensitive in situ RNA detection using Yn-situ.
Wu Y, Xu W, Ma L, Yu Z, Wang Y, Yu CR. Wu Y, et al. Cell Rep Methods. 2022 Apr 18;2(4):100201. doi: 10.1016/j.crmeth.2022.100201. eCollection 2022 Apr 25. Cell Rep Methods. 2022. PMID: 35497500 Free PMC article.
We describe a cost-effective, highly sensitive, and quantitative method for in situ detection of RNA molecules in tissue sections. This method, dubbed Yn-situ, standing for Y-branched probe in situ hybridization, uses a single-strand DNA preamplifier w …
We describe a cost-effective, highly sensitive, and quantitative method for in situ detection of RNA molecules in tissue secti …
Protocol to assess RNA-RNA interactions in situ using an RNA-proximity ligation assay.
Basavappa MG, Henao-Mejia J, Cherry S. Basavappa MG, et al. STAR Protoc. 2022 Dec 16;3(4):101892. doi: 10.1016/j.xpro.2022.101892. Epub 2022 Dec 1. STAR Protoc. 2022. PMID: 36595913 Free PMC article.
Here, we describe a protocol to assess RNA-RNA interactions in situ using an adapted proximity ligation assay (PLA). We detail steps to perform RNA-probe hybridization, in situ rolling circle amplification, and immunofluorescence confocal micros …
Here, we describe a protocol to assess RNA-RNA interactions in situ using an adapted proximity ligation assay (PLA). We …
Intracellular RNA-tracking methods.
George L, Indig FE, Abdelmohsen K, Gorospe M. George L, et al. Open Biol. 2018 Oct 3;8(10):180104. doi: 10.1098/rsob.180104. Open Biol. 2018. PMID: 30282659 Free PMC article. Review.
RNA tracking allows researchers to visualize RNA molecules in cells and tissues, providing important spatio-temporal information regarding RNA dynamics and function. ...Here, we discuss the mechanisms, advantages, and weaknesses of in situ hybridizatio …
RNA tracking allows researchers to visualize RNA molecules in cells and tissues, providing important spatio-temporal informati …
GeoMx RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue.
Zollinger DR, Lingle SE, Sorg K, Beechem JM, Merritt CR. Zollinger DR, et al. Methods Mol Biol. 2020;2148:331-345. doi: 10.1007/978-1-0716-0623-0_21. Methods Mol Biol. 2020. PMID: 32394392
RNA in situ hybridization (ISH) is a widely used technique for the localization of mRNA in tissues. ...Cleaved indices are then quantified using NanoString nCounter() Technology generating digital quantification of RNA expression with spatial context....
RNA in situ hybridization (ISH) is a widely used technique for the localization of mRNA in tissues. ...Cleaved indices are the …
Whole-mount RNA in situ hybridization technique in Torenia ovules.
Su S, Zhou X, Higashiyama T. Su S, et al. Plant Reprod. 2023 Jun;36(2):139-146. doi: 10.1007/s00497-022-00455-2. Epub 2022 Dec 21. Plant Reprod. 2023. PMID: 36543964
The expression pattern of an interested gene at a cellular level provides strong evidence for its functions. RNA in situ hybridization has been proved to be a powerful tool in detecting the spatial-temporal expression pattern of a gene in various organisms. However, …
The expression pattern of an interested gene at a cellular level provides strong evidence for its functions. RNA in situ hybri …
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