Preparation and partial structural characterization of the exopolysaccharide from Bacillus mucilaginosus SM-01

Carbohydr Polym. 2016 Aug 1:146:217-23. doi: 10.1016/j.carbpol.2016.03.038. Epub 2016 Mar 19.

Abstract

In this study, an acidic hetero-exopolysaccharide, designated as BMPS, was isolated from the culture supernatant of bacteria Bacillus mucilaginosus SM-01 by ion-exchange and size-exclusion chromatography. The molecular weight of BMPS was measured to be 2.67×10(6)Da. To analyze the structure of the polysaccharide, the low-molecular weight fragment (BMPS-H) of BMPS was obtained under mild hydrolysis. BMPS-H was determined to be a liner neutral fraction with an average molecular weight of 1621Da. Monosaccharide analysis indicated that BMPS-H contained glucose and mannose in a molar ratio of 1.5:1. Based on methylation analysis and NMR spectroscopy data, the following structure of BMPS-H was established: In addition, MALDI-TOF-MS analysis showed the hydroxyl group on C-2 of 1,4-linked-glucosyl residues was partially acetylated.

Keywords: Bacillus mucilaginosus; Exopolysaccharide; Methylation analysis; Nuclear magnetic resonance (NMR) spectroscopy; Structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / chemistry*
  • Chromatography, Gel
  • Hydrolysis
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Molecular Weight
  • Polysaccharides, Bacterial / chemistry*
  • Polysaccharides, Bacterial / isolation & purification

Substances

  • Polysaccharides, Bacterial