Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens.
Nimsamer P, Mayuramart O, Rattanaburi S, Chantaravisoot N, Saengchoowong S, Puenpa J, Poovorawan Y, Payungporn S.
Nimsamer P, et al.
J Virol Methods. 2021 Apr;290:114092. doi: 10.1016/j.jviromet.2021.114092. Epub 2021 Feb 1.
J Virol Methods. 2021.
PMID: 33539846
Free PMC article.
The assays with E, N2 and S genes yielded acceptable sensitivity of detection ( 95 %) whereas N1 and S genes provided outstanding specificity of detection (100 %). Preferably, multiple target genes should be detected by using CRISPR-Cas12a to ensure the most effecti …
The assays with E, N2 and S genes yielded acceptable sensitivity of detection ( 95 %) whereas N1 and S genes provided outstand …