Abstract
[reaction: see text] Fluorescence can be quenched through PET from the fluorophore to neighboring C, T, and G bases, but not to the A moiety. The hairpin stem stability arising from pi-stacking and the PET between the pyrene-labeled 2'-deoxynucleotide units and their neighboring bases are the two main factors that affect the operation of these novel fluorescent oligonucleotides.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Pair Mismatch*
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Base Sequence
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DNA / analysis
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DNA / chemistry*
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Fluorescence Resonance Energy Transfer
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Fluorescent Dyes / chemical synthesis*
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Fluorescent Dyes / chemistry
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Molecular Sequence Data
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Oligonucleotide Probes / chemical synthesis*
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Oligonucleotide Probes / chemistry
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Pyrenes / chemistry
Substances
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Fluorescent Dyes
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Oligonucleotide Probes
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Pyrenes
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DNA