Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics.
Leppek K, Byeon GW, Kladwang W, Wayment-Steele HK, Kerr CH, Xu AF, Kim DS, Topkar VV, Choe C, Rothschild D, Tiu GC, Wellington-Oguri R, Fujii K, Sharma E, Watkins AM, Nicol JJ, Romano J, Tunguz B, Diaz F, Cai H, Guo P, Wu J, Meng F, Shi S, Participants E, Dormitzer PR, Solórzano A, Barna M, Das R.
Leppek K, et al.
Nat Commun. 2022 Mar 22;13(1):1536. doi: 10.1038/s41467-022-28776-w.
Nat Commun. 2022.
PMID: 35318324
Free PMC article.
We further introduce a method called In-line-seq, applied to thousands of diverse RNAs, that reveals sequence and structure-based rules for mitigating hydrolytic degradation. Our findings show that highly structured "superfolder" mRNAs can be designed to improve both stabi …
We further introduce a method called In-line-seq, applied to thousands of diverse RNAs, that reveals sequence and structure-based rules …