Objective: To study the plasma protein binding rate of Tetramethylpyrazine Phosphate.
Methods: The ultrafiltration was employed to determine the plasma protein binding rate of Tetramethylpyrazine Phosphate. The plasma concentrations of Tetramethylpyrazine Phosphate were measured by RP-HPLC.
Results: The plasma protein binding rate of Tetramethylpyrazine Phosphate with HSA, human plasma and rat plasma were (64.64 +/- 0.68)%, (65.85 +/- 7.35)% and (73.65 +/- 2.35)%, respectively.
Conclusion: The binding rate of Tetramethylpyrazine Phosphate with plasma protein is middling strength.