[Determination of plasma protein binding rate of tetramethylpyrazine phosphate by ultrafiltration]

Zhong Yao Cai. 2010 Aug;33(8):1282-5.
[Article in Chinese]

Abstract

Objective: To study the plasma protein binding rate of Tetramethylpyrazine Phosphate.

Methods: The ultrafiltration was employed to determine the plasma protein binding rate of Tetramethylpyrazine Phosphate. The plasma concentrations of Tetramethylpyrazine Phosphate were measured by RP-HPLC.

Results: The plasma protein binding rate of Tetramethylpyrazine Phosphate with HSA, human plasma and rat plasma were (64.64 +/- 0.68)%, (65.85 +/- 7.35)% and (73.65 +/- 2.35)%, respectively.

Conclusion: The binding rate of Tetramethylpyrazine Phosphate with plasma protein is middling strength.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apiaceae / chemistry
  • Blood Proteins / metabolism*
  • Chromatography, High Pressure Liquid
  • Humans
  • Phosphates / metabolism*
  • Protein Binding
  • Pyrazines / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Sensitivity and Specificity
  • Ultrafiltration / methods*
  • Vasodilator Agents / metabolism*

Substances

  • Blood Proteins
  • Phosphates
  • Pyrazines
  • Vasodilator Agents
  • tetramethylpyrazine