Insights into the antibacterial activity and antibacterial mechanism of silver modified fullerene towards Staphylococcus aureus by multiple spectrometric examinations

Yin-Xu Pan; Qiu-Hui Xu; Hua-Ming Xiao; Chun-Ya Li

doi:10.1016/j.chemosphere.2023.140136

Insights into the antibacterial activity and antibacterial mechanism of silver modified fullerene towards Staphylococcus aureus by multiple spectrometric examinations

Chemosphere. 2023 Nov:342:140136. doi: 10.1016/j.chemosphere.2023.140136. Epub 2023 Sep 10.

Authors

Yin-Xu Pan¹, Qiu-Hui Xu², Hua-Ming Xiao³, Chun-Ya Li⁴

Affiliations

¹ Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central Minzu University, Wuhan, 430074, China.
² Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
³ Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central Minzu University, Wuhan, 430074, China; Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. Electronic address: xiaohuaming@caas.cn.
⁴ Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central Minzu University, Wuhan, 430074, China. Electronic address: lichychem@mail.scuec.edu.cn.

PMID: 37699456
DOI: 10.1016/j.chemosphere.2023.140136

Abstract

Clarifying the antibacterial mechanism of silver (Ag)-based materials is of great significance for the rational design, synthesis, and evaluation of antimicrobials. Herein, detailed description of the antibacterial mechanism of a synthesized silver deposited fullerene material (Ag(I)-C₆₀) towards Staphylococcus aureus was surveyed from the point of view of DNA damage by ultraviolet-visible spectroscopy (UV-vis), inductively coupled plasma mass spectrometry (ICP-MS), and liquid chromatography-mass spectrometry (LC-MS). The model material, Ag(I)-C₆₀, was prepared by liquid-liquid interfacial precipitation method, and characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), thermos-gravimetric analysis (TGA), and nitrogen adsorption/desorption analysis. Ultra-efficient bacteriostatic rate of Ag(I)-C₆₀ was found to be 88.98% under light irradiation for 20 min. UV-vis measurement of the composition changes of four DNA bases showed that they changed in the presence of Ag(I)-C₆₀ under light irradiation, suggesting Ag(I)-C₆₀ could destroy the cells and genetic material of Staphylococcus aureus and thereby inhibit its growth and reproduction. ICP-MS analysis demonstrated the releasing behavior of Ag⁺ from Ag-based materials. Finally, the transformation pathway of G, A, C, and T were measured by LC-MS, demonstrating the conversion of Adenine (m/z 136.06) to 8-OH-Ade (m/z 174.04). These collective results suggested that Ag(I)-C₆₀ was a new ultra-efficient antibacterial by slowly releasing Ag⁺ in water and producing a large amount of ROS under light.

Keywords: Antibacterial; DNA damage; Fullerene material; Mass spectrometry; Silver based material; Transformation pathway.