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Expression, purification, and characterization of equine lactoferrin in Pichia pastoris.
Paramasivam M, Saravanan K, Uma K, Sharma S, Singh TP, Srinivasan A. Paramasivam M, et al. Protein Expr Purif. 2002 Oct;26(1):28-34. doi: 10.1016/s1046-5928(02)00528-4. Protein Expr Purif. 2002. PMID: 12356467
In the present study, we report the production of recombinant equine lactoferrin (ELF) in the methylotropic yeast Pichia pastoris using pPIC9K vector. The recombinant protein was purified by one-step affinity chromatography using heparin-Sepharose column. ...
In the present study, we report the production of recombinant equine lactoferrin (ELF) in the methylotropic yeast Pichia pastoris usi …
Biotinyl endothelin-1 binding to endothelin receptor and its applications.
Saravanan K, Paramasivam M, Dey S, Singh TP, Srinivasan A. Saravanan K, et al. J Cardiovasc Pharmacol. 2004 Sep;44(3):287-93. doi: 10.1097/01.fjc.0000132316.24513.ec. J Cardiovasc Pharmacol. 2004. PMID: 15475824
This method is applicable to detergent solubilized receptors and purified recombinant receptors. The endothelin receptor type A expressed in Pichia pastoris system has been used in this study. We show that this method is applicable in Western blot analysis of endothelin-1 …
This method is applicable to detergent solubilized receptors and purified recombinant receptors. The endothelin receptor type A expressed in …
Endothelin and its receptor interactions: role of extracellular receptor domain and length of peptide ligands.
Saravanan K, Hariprasad G, Jitesh O, Das U, Dey S, Sharma S, Kaur P, Singh TP, Srinivasan A. Saravanan K, et al. Protein Pept Lett. 2007;14(8):779-83. doi: 10.2174/092986607781483651. Protein Pept Lett. 2007. PMID: 17979818
Human endothelin B receptor and its domain-truncated forms were cloned and expressed in Pichia pastoris. Ligand binding studies with expressed proteins were carried out using biotinylated endothelins. ...
Human endothelin B receptor and its domain-truncated forms were cloned and expressed in Pichia pastoris. Ligand binding studies with …