Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells

Manuela Ocaña-Macchi; Meret E Ricklin; Sylvie Python; Gsell-Albert Monika; Jürgen Stech; Olga Stech; Artur Summerfield

doi:10.1016/j.virol.2012.01.037

Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells

Virology. 2012 May 25;427(1):1-9. doi: 10.1016/j.virol.2012.01.037. Epub 2012 Feb 23.

Authors

Manuela Ocaña-Macchi¹, Meret E Ricklin, Sylvie Python, Gsell-Albert Monika, Jürgen Stech, Olga Stech, Artur Summerfield

Affiliation

¹ Institute of Virology and Immunoprophylaxis, Mittelhäusern, Switzerland.

PMID: 22365327
DOI: 10.1016/j.virol.2012.01.037

Abstract

The 2009 influenza A virus (IAV) pandemic resulted from reassortment of avian, human and swine strains probably in pigs. To elucidate the role of viral genes in host adaptation regarding innate immune responses, we focussed on the effect of genes from an avian H5N1 and a porcine H1N1 IAV on infectivity and activation of porcine GM-CSF-induced dendritic cells (DC). The highest interferon type I responses were achieved by the porcine virus reassortant containing the avian polymerase gene PB2. This finding was not due to differential tropism since all viruses infected DC equally. All viruses equally induced MHC class II, but porcine H1N1 expressing the avian viral PB2 induced more prominent nuclear NF-κB translocation compared to its parent IAV. The enhanced activation of DC may be detrimental or beneficial. An over-stimulation of innate responses could result in either pronounced tissue damage or increased resistance against IAV reassortants carrying avian PB2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Birds
Cell Line
Chick Embryo
Dendritic Cells / immunology
Dendritic Cells / virology*
Dogs
Genes, Viral
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
Host-Pathogen Interactions*
Humans
Influenza A Virus, H1N1 Subtype / genetics
Influenza A Virus, H1N1 Subtype / immunology*
Influenza A Virus, H5N1 Subtype / genetics
Influenza A Virus, H5N1 Subtype / immunology*
Interferon Type I / metabolism*
Major Histocompatibility Complex
Mice
NF-kappa B / metabolism
Orthomyxoviridae Infections / immunology*
Orthomyxoviridae Infections / veterinary
Orthomyxoviridae Infections / virology
Pandemics
Promoter Regions, Genetic / genetics
RNA-Dependent RNA Polymerase / immunology*
Reassortant Viruses / genetics
Reassortant Viruses / immunology*
Swine
Swine Diseases / immunology
Swine Diseases / virology*
Viral Proteins / immunology*
Virus Replication / genetics

Substances

Interferon Type I
NF-kappa B
PB2 protein, Influenzavirus A
Viral Proteins
Granulocyte-Macrophage Colony-Stimulating Factor
RNA-Dependent RNA Polymerase