Determination of 21 antibiotics in sea cucumber using accelerated solvent extraction with in-cell clean-up coupled to ultra-performance liquid chromatography-tandem mass spectrometry

Minghua Zhu; Hongxia Zhao; Deming Xia; Juan Du; Huaijun Xie; Jingwen Chen

doi:10.1016/j.foodchem.2018.03.051

Determination of 21 antibiotics in sea cucumber using accelerated solvent extraction with in-cell clean-up coupled to ultra-performance liquid chromatography-tandem mass spectrometry

Food Chem. 2018 Aug 30:258:87-94. doi: 10.1016/j.foodchem.2018.03.051. Epub 2018 Mar 14.

Authors

Minghua Zhu¹, Hongxia Zhao², Deming Xia³, Juan Du³, Huaijun Xie³, Jingwen Chen⁴

Affiliations

¹ Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. Electronic address: minghuazhu@mail.dlut.edu.cn.
² Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. Electronic address: hxzhao@dlut.edu.cn.
³ Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
⁴ Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. Electronic address: jwchen@dlut.edu.cn.

PMID: 29655759
DOI: 10.1016/j.foodchem.2018.03.051

Abstract

An accelerated solvent extraction (ASE) with in-cell clean-up method coupled to ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to determine 21 antibiotics in sea cucumber. The analytes include 10 sulfonamides, 4 fluoroquinolones, 3 amphenicols, 2 beta-lactams, 1 lincosamide and trimethoprim. Optimal parameters of ASE method were obtained at 80 °C, 1 static cycle of 5 min with methanol/acetonitrile (1/1, v/v) using 2 g of C18 as adsorbent. Recoveries at 50.1-129.2% were achieved with RSD under 20%. Method detection limits ranged from 0.03 to 2.9 μg kg^-1. Compared to the reported ultrasound-assisted extraction method, the proposed method offered comparable extraction efficiency for sulfonamides from sea cucumber, but higher for other categories of antibiotics. This validated method was then successfully applied to sea cucumber samples and 9 antibiotics were detected with the highest concentration up to 57.7 μg kg^-1 for norfloxacin.

Keywords: ASE; Antibiotic; Chloramphenicol (PubChem CID: 5959); Ciprofloxacin (PubChem CID: 2764); Enrofloxacin (PubChem CID: 71188); In-cell clean-up; Penicillin G (PubChem CID: 21668834); Sea cucumber; Sulfadimethoxine (PubChem CID: 5321); Sulfamethazine (PubChem CID: 5327); Sulfathiazole (PubChem CID: 5340); Trimethoprim (PubChem CID: 5578); UPLC-MS/MS.

MeSH terms

Animals
Anti-Bacterial Agents / analysis*
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / isolation & purification
Chromatography, High Pressure Liquid*
Fluoroquinolones / analysis
Fluoroquinolones / chemistry
Fluoroquinolones / isolation & purification
Limit of Detection
Sea Cucumbers / chemistry*
Sea Cucumbers / metabolism
Solid Phase Extraction
Sulfonamides / analysis
Sulfonamides / chemistry
Sulfonamides / isolation & purification
Tandem Mass Spectrometry*
beta-Lactams / analysis
beta-Lactams / chemistry
beta-Lactams / isolation & purification

Substances

Anti-Bacterial Agents
Fluoroquinolones
Sulfonamides
beta-Lactams