Isoquinolines from national herb Corydalis tomentella and neuroprotective effect against lipopolysaccharide-induced BV2 microglia cells

Bioorg Chem. 2020 Jan:95:103489. doi: 10.1016/j.bioorg.2019.103489. Epub 2019 Dec 9.

Abstract

Five new isoquinolines (1-5) were isolated from national herb Corydalis tomentella. Their structures were elucidated by extensive analysis of the 1D and 2D NMR spectra and from the HRESIMS. Absolute configurations of 1-3 were determined by comparing their experimental and computed ECD data. Since plants from Corydalis have been reported to protect against Alzheimer's disease, all compounds were evaluated for their neuroprotective effect against lipopolysaccharide-induced BV2 microglia cells. Compound 2 and 3 showed well anti-neuroinflammatory activity at low concentration (25 μM).

Keywords: Corydalis tomentella; In vitro; Isoquinolines; Neuroprotective effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / drug therapy*
  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Corydalis / chemistry*
  • Density Functional Theory
  • Dose-Response Relationship, Drug
  • Isoquinolines / chemistry
  • Isoquinolines / isolation & purification
  • Isoquinolines / pharmacology*
  • Lipopolysaccharides / antagonists & inhibitors*
  • Lipopolysaccharides / pharmacology
  • Mice
  • Microglia / drug effects*
  • Molecular Structure
  • Neuroprotective Agents / chemistry
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology*
  • Structure-Activity Relationship

Substances

  • Isoquinolines
  • Lipopolysaccharides
  • Neuroprotective Agents