Effect of laser radiation with wave length 1,4 microm on exposed cornea of isolated bull eye (in vitro) is studied. Experimental laser coagulator with wave length 1,4 microm based on the semiconductor laser with a fiber radiation output was used in the study. Cornea was exposed at the standardized distance to radiation with different energy settings. Coagulates were morphologically studied, photoregistration and morphometric analysis of obtained images were performed. The depth of radiation absorbtion, type and degree of corneal damage were estimated in the focus and marginal area. The analysis of results shows potential of this laser coagulator to be used for selective effects at different corneal layers. This laser may be applied both for coagulation and therapueutic purposes.