In vitro antifungal and antibiofilm activities of novel sulfonyl hydrazone derivatives against Candida spp

Merve Aydin; Ali Ozturk; Tugce Duran; Ummuhan Ozdemir Ozmen; Esra Sumlu; Esra Bilen Ayan; Emine Nedime Korucu

doi:10.1016/j.mycmed.2022.101327

In vitro antifungal and antibiofilm activities of novel sulfonyl hydrazone derivatives against Candida spp

J Mycol Med. 2023 Mar;33(1):101327. doi: 10.1016/j.mycmed.2022.101327. Epub 2022 Aug 18.

Authors

Merve Aydin¹, Ali Ozturk², Tugce Duran³, Ummuhan Ozdemir Ozmen⁴, Esra Sumlu⁵, Esra Bilen Ayan⁴, Emine Nedime Korucu⁶

Affiliations

¹ Department of Medical Microbiology, Faculty of Medicine, KTO Karatay University, Konya, Turkey. Electronic address: merve.aydin@karatay.edu.tr.
² Department of Medical Microbiology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde, Turkey.
³ Department of Medical Genetics, Faculty of Medicine, KTO Karatay University, Konya, Turkey.
⁴ Department of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey.
⁵ Department of Medical Pharmacology, Faculty of Medicine, KTO Karatay University, Konya, Turkey.
⁶ Department of Molecular Biology and Genetics, Faculty of Science, Necmettin Erbakan University, Konya, Turkey.

PMID: 36272382
DOI: 10.1016/j.mycmed.2022.101327

Abstract

Background: The aim of this study was to investigate the antifungal and antibiofilm activity of the new sulfonyl hydrazones compound derived from sulphonamides.

Methods: In this study, new sulfonyl hydrazone series were synthesized via a green chemistry method. The structures of the synthesized compounds were characterized by elemental analyses and spectroscopic methods. The antifungal activities of the Anaf compounds against Candida strains under planktonic conditions were tested. The biofilm-forming ability of Candida strains was determined and the inhibitory effects of Anaf compounds on Candida biofilms compared with fluconazole were measured by MTT assay. Expression analysis of biofilm-related genes was investigated with qRT-PCR. The statistical analysis was performed using a one-way ANOVA test. CANDIDA: strains was determined and the inhibitory effects of Anaf compounds on Candida biofilms compared with fluconazole were measured by MTT assay. Expression analysis of biofilm-related genes was investigated with qRT-PCR. The statistical analysis was performed using a one-way ANOVA test.

Results: A total of 16 (45.7%) out of 35 Candida isolates were determined as strong biofilm producers in this study. C. albicans was the most biofilm producer, followed by C. krusei and C. lusitaniae. The Anaf compounds had a broad spectrum of activity with MIC values ranging from 4 μg/ml to 64 μg/ml. Our data indicated that the Anaf compound had a significant effect on inhibiting biofilm formation in both fluconazole-susceptible and -resistant strains. The expression levels of hypha-specific genes als3, hwp1, ece1 and sap5 were downregulated by Anaf compounds.

Conclusions: Our study revealed that the Anaf compounds had antifungal activity and inhibited fungal biofilms, which may be related to the suppression of C. albicans adherence and hyphal formation. These results suggest that Anaf compounds may have therapeutic potential for the treatment and prevention of biofilm-associated Candida infections.

Keywords: Antibiofilm; Antifungal; Biofilm; Biofilm-related genes; Candida; New sulfonyl hydrazone.

MeSH terms

Antifungal Agents* / pharmacology
Biofilms
Candida albicans
Candida*
Fluconazole / pharmacology
Microbial Sensitivity Tests

Substances

Antifungal Agents
Fluconazole