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Screening for protease substrate by polyvalent phage display.
Sedlacek R, Chen E. Sedlacek R, et al. Comb Chem High Throughput Screen. 2005 Mar;8(2):197-203. doi: 10.2174/1386207053258541. Comb Chem High Throughput Screen. 2005. PMID: 15777183
Traditional methods for establishing substrate recognition profiles involve the identification of the scissile bond within a given protein substrate by proteomic methods such as Edman degradation. Then, synthetic peptide variants of this sequence can be screened in an iter …
Traditional methods for establishing substrate recognition profiles involve the identification of the scissile bond within a given protein s …
Site-specific semisynthetic variant of human hemoglobin.
Hefta SA, Lyle SB, Busch MR, Harris DE, Matthew JB, Gurd FR. Hefta SA, et al. Proc Natl Acad Sci U S A. 1988 Feb;85(3):709-13. doi: 10.1073/pnas.85.3.709. Proc Natl Acad Sci U S A. 1988. PMID: 3422453 Free PMC article.
A single round of Edman degradation was employed to remove the NH2-terminal valine from isolated alpha chains of human hemoglobin. ...
A single round of Edman degradation was employed to remove the NH2-terminal valine from isolated alpha chains of human hemoglobin. .. …