A new method was developed and validated for the determination of 2-(4-fluorophenyl)-5-methylsulfonyl-1,3,4-oxadiazole (jiahuangxianjunzuo, JHXJZ) by ultra-performance liquid chromatography equipped with photo-diode array detector. JHXJZ from tomato and soil was extracted with ethyl acetate without further cleanup. The limits of detection and quantification of JHXJZ were 0.0083 and 0.025 mg kg(-1) in tomato, 0.0017 and 0.005 mg kg(-1) in soil, respectively. The average recoveries of tomato and soil were studied at three spiked levels and ranged from 84.51 % to 101.30 % and 85.30 % to 101.53 %, respectively, with relative standard deviations of 2.61 %-4.13 % and 1.21 %-4.80 %, respectively. The results indicated that the reported method could meet the requirement for the analysis of JHXJZ in trace amount in tomato and soil.