Abstract
We determined the complete nucleotide sequence of an infectious bursal disease (IBD) virus (IBDV) isolate (designated KZC-104) from a confirmed IBD outbreak in Lusaka in 2004. The genome consisted of 3,074 and 2,651 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segment A of KZC-104 was derived from a very virulent (VV) strain, whereas its segment B was derived from a classical attenuated strain. On BLAST search, the full-length segment A and B sequences showed 98 % nucleotide sequence identity to the VV strain D6948 and 99.8 % nucleotide sequence identity to the classical attenuated strain D78. This is a unique IBDV reassortant strain that has emerged in nature, involving segment B of a cell-culture-adapted attenuated vaccine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Birnaviridae Infections / veterinary*
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Birnaviridae Infections / virology
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Chickens*
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Disease Outbreaks / veterinary
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Genome, Viral*
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Infectious bursal disease virus / classification
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Infectious bursal disease virus / genetics*
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Infectious bursal disease virus / isolation & purification
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Infectious bursal disease virus / pathogenicity
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Molecular Sequence Data
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Open Reading Frames
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Phylogeny
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Poultry Diseases / virology*
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Reassortant Viruses / genetics*
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Reassortant Viruses / isolation & purification
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Reassortant Viruses / pathogenicity
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Sequence Alignment
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Sequence Analysis, DNA
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Viral Nonstructural Proteins / chemistry
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Viral Nonstructural Proteins / genetics
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Viral Structural Proteins / chemistry
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Viral Structural Proteins / genetics
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Zambia
Substances
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VP1 protein, infectious bursal disease virus
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VP5 protein, infectious bursal disease virus
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Viral Nonstructural Proteins
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Viral Structural Proteins
Associated data
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GENBANK/AB368968
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GENBANK/AB368969