Abstract
Structural studies by in-cell nuclear magnetic resonance are a developing new field of research, and their objective is to obtain structural information of proteins and other biological macromolecules in the cytoplasm of Escherichia coli cells. The major limitation of in-cell experiments is cell lysis that occurs during the experiments. In this article, we describe how inhibition of autologous expression by rifampicin at a high concentration decreases cell lysis in E. coli. We suggest that rifampicin is acting in the programmed cell death gene system MazEF, which is triggered by stress conditions and ultimately leads to cell lysis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriolysis / drug effects
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Bacteriolysis / physiology*
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Cell Death / drug effects
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Cell Death / physiology
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Cytoplasm / metabolism
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DNA-Binding Proteins / drug effects
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DNA-Binding Proteins / metabolism
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Escherichia coli / cytology
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Escherichia coli / drug effects*
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Escherichia coli / metabolism*
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Escherichia coli Proteins / antagonists & inhibitors*
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Escherichia coli Proteins / drug effects
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Escherichia coli Proteins / metabolism
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Gene Expression Regulation, Bacterial / drug effects
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Genes, Bacterial / drug effects
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Nuclear Magnetic Resonance, Biomolecular / methods*
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Rifampin / pharmacology
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Thioredoxins / analysis
Substances
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DNA-Binding Proteins
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Escherichia coli Proteins
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MazE protein, E coli
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Thioredoxins
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Rifampin