An in vivo platform to select and evolve aggregation-resistant proteins.
Ebo JS, Saunders JC, Devine PWA, Gordon AM, Warwick AS, Schiffrin B, Chin SE, England E, Button JD, Lloyd C, Bond NJ, Ashcroft AE, Radford SE, Lowe DC, Brockwell DJ.
Ebo JS, et al.
Nat Commun. 2020 Apr 14;11(1):1816. doi: 10.1038/s41467-020-15667-1.
Nat Commun. 2020.
PMID: 32286330
Free PMC article.
Using this assay as a directed evolution screen, we demonstrate the generation of aggregation resistant scFv sequences when reformatted as IgGs. This powerful tool can thus screen and evolve 'manufacturable' biopharmaceuticals early in industrial development. ...
Using this assay as a directed evolution screen, we demonstrate the generation of aggregation resistant scFv sequences when reformatted as …