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An improved method for increasing the efficiency of gene transfection and transduction.
Shi B, Xue M, Wang Y, Wang Y, Li D, Zhao X, Li X. Shi B, et al. Int J Physiol Pathophysiol Pharmacol. 2018 Apr 20;10(2):95-104. eCollection 2018. Int J Physiol Pathophysiol Pharmacol. 2018. PMID: 29755642 Free PMC article.
Here we compared the transfection and viral transduction efficiency using commercially available transfection reagents including FuGENE 6, Lipofectamine 2000 and Lipofectamine 3000 in different cell lines and primary cultured cells. ...Western blot showed the strongest EGF …
Here we compared the transfection and viral transduction efficiency using commercially available transfection reagents including FuGENE …
Tumor necrosis factor α stimulates Her-2 cleavage by activated caspase-8.
Li X, Zhao Y, Zhang Y, Du N, Ren H. Li X, et al. Cell Physiol Biochem. 2012;30(4):889-97. doi: 10.1159/000341466. Epub 2012 Aug 27. Cell Physiol Biochem. 2012. PMID: 22922291 Free article.
METHODS: Stably transfected MCF-7 cell lines with pcDNA3.0, IqBalpha MT, c-FLIP/control shRNA were established by FuGENE with the supplementation of G418 (500 g /ml). Western blot and Real-time PCR were applied to assess the expression levels of protein and mRNA of target …
METHODS: Stably transfected MCF-7 cell lines with pcDNA3.0, IqBalpha MT, c-FLIP/control shRNA were established by FuGENE with the sup …
The establishment of clonally derived chicken embryonic fibroblast cell line (CSC) with high transfection efficiency and ability as a feeder cell.
Zhao R, Jin J, Sun X, Jin K, Wang M, Ahmed MF, Zuo Q, Zhang Y, Zhao Z, Chen G, Li B. Zhao R, et al. J Cell Biochem. 2018 Nov;119(11):8841-8850. doi: 10.1002/jcb.27137. Epub 2018 Aug 4. J Cell Biochem. 2018. PMID: 30076744
CSC has a normal chicken diploid karyotype, no tumorigenicity, and a high G2/M phase cell ratio. We found that Fugene could mediate the transfection of CSCs efficiently; it was significantly improved compared with the primary cells. ...
CSC has a normal chicken diploid karyotype, no tumorigenicity, and a high G2/M phase cell ratio. We found that Fugene could mediate t …
Establishment of human embryonic stem cell line stably expressing Epstein-Barr virus-encoded nuclear antigen 1.
Ren CP, Zhao M, Shan WJ, Yang XY, Yin ZH, Jiang XJ, Zhang HB, Yao KT. Ren CP, et al. Acta Biochim Biophys Sin (Shanghai). 2005 Jan;37(1):68-73. doi: 10.1093/abbs/37.1.68. Acta Biochim Biophys Sin (Shanghai). 2005. PMID: 15645084
Therefore, the first step was to establish EBNA1-positive hES cells. Using the Fugene 6 transfection reagent, we transfected hES cells with the EBNA1 expression vector and subsequently generated hES cell clones that stably expressed EBNA1 under drug selection. ...
Therefore, the first step was to establish EBNA1-positive hES cells. Using the Fugene 6 transfection reagent, we transfected hES cell …
Superparamagnetic iron oxide labeling of spinal cord neural stem cells genetically modified by nerve growth factor-beta.
Lei D, Zhao H, Deng X, Liu R, Zhang F, Yao D. Lei D, et al. J Huazhong Univ Sci Technolog Med Sci. 2009 Apr;29(2):235-8. doi: 10.1007/s11596-009-0220-3. Epub 2009 Apr 28. J Huazhong Univ Sci Technolog Med Sci. 2009. PMID: 19399412
The cells of the third passage were transfected with plasmid pcDNA3-hNGFbeta by using FuGENE HD transfection reagent. The expression of NGF-beta was measured by immunocytochemistry and Western blotting. The positive clones were selected, allowed to proliferate and then lab …
The cells of the third passage were transfected with plasmid pcDNA3-hNGFbeta by using FuGENE HD transfection reagent. The expression …
The neuroprotective role of TERT via an antiapoptotic mechanism in neonatal rats after hypoxia-ischemia brain injury.
Zhao F, Qu Y, Xiong T, Duan Z, Ye Q, Mu D. Zhao F, et al. Neurosci Lett. 2012 Apr 25;515(1):39-43. doi: 10.1016/j.neulet.2012.03.014. Epub 2012 Mar 15. Neurosci Lett. 2012. PMID: 22446191
Plasmid containing mock, TERT antisense or sense fragment mixed with Fugene HD was injected to the right lateral ventricle immediately after the insult respectively. ...
Plasmid containing mock, TERT antisense or sense fragment mixed with Fugene HD was injected to the right lateral ventricle immediatel …