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Construction of the ie1-Bacmid expression system and its use to express EGFP and BmAGO2 in BmN cells.
Zhou F, Gao Z, Lv Z, Chen J, Hong Y, Yu W, Wang D, Jiang C, Wu X, Zhang Y, Nie Z. Zhou F, et al. Appl Biochem Biotechnol. 2013 Apr;169(8):2237-47. doi: 10.1007/s12010-013-0137-y. Epub 2013 Feb 24. Appl Biochem Biotechnol. 2013. PMID: 23436226
In this work, we constructed and screened a eukaryotic expression vector for silkworm cells The EGFP and B. mori Argonaute2 proteins were found to be efficiently expressed using the screened pIEx-1 vector with the FuGENE 6 transfection reagent. Additionally, we constructed …
In this work, we constructed and screened a eukaryotic expression vector for silkworm cells The EGFP and B. mori Argonaute2 proteins were fo …
The piggyBac transposon is an integrating non-viral gene transfer vector that enhances the efficiency of GDEPT.
Kang Y, Zhang XY, Jiang W, Wu CQ, Chen CM, Gu JR, Zheng YF, Xu CJ. Kang Y, et al. Cell Biol Int. 2009 Apr;33(4):509-15. doi: 10.1016/j.cellbi.2009.01.017. Cell Biol Int. 2009. PMID: 19353779
The recombinant plasmid, pPB/TK, was transfected into ovarian adenocarcinoma cells SKOV3 with FuGENE HD reagent, and the efficiency was given by the percentage of mRFP1-positive cells detected by flow cytometry and confocal microscopy. ...
The recombinant plasmid, pPB/TK, was transfected into ovarian adenocarcinoma cells SKOV3 with FuGENE HD reagent, and the efficiency w …
PhiC31 integrase-mediated genomic integration and stable gene expression in the mouse mammary gland after gene electrotransfer.
Luo Y, Liu J, Wang Y, Su J, Wu Y, Hu G, Gao M, Quan F, Zhang Y. Luo Y, et al. J Gene Med. 2013 Oct;15(10):356-65. doi: 10.1002/jgm.2723. J Gene Med. 2013. PMID: 24288809
METHODS: The normal mouse mammary epithelial cell line HC11 was transfected with FuGENE HD Transfection Reagent (Roche Diagnostics, Shanghai, China). ...
METHODS: The normal mouse mammary epithelial cell line HC11 was transfected with FuGENE HD Transfection Reagent (Roche Diagnostics, S …
[Long-term and stable expression of transgene mediated by piggyBac transposon in gynecological malignant tumor cells].
Kang Y, Sun QW, Yu WB, Cheng MJ, Zhang XY, Wu X, Chen CM, Zheng YF, Xu CJ. Kang Y, et al. Zhonghua Fu Chan Ke Za Zhi. 2010 Apr;45(4):292-7. Zhonghua Fu Chan Ke Za Zhi. 2010. PMID: 20646543 Chinese.
METHODS: Amplified herpes simplex virus thymidine kinase (HSV-tk) gene coding region by PCR and integrated it into PB expression vector, PB[Act-RFP]DS, for reconstructing PB[Act-RFP, HSV-tk]DS (pPB/TK). By using different transfection reagents: FuGENE HD, jetPEI, lipofecta …
METHODS: Amplified herpes simplex virus thymidine kinase (HSV-tk) gene coding region by PCR and integrated it into PB expression vector, PB[ …
[Antitumor effect of gene gun-mediated DNA vaccine pWRG-neu immunization in C57BL/6 mice].
Zha YY, Lin C, Zhang SR, Liang X, Zhang XY, Fu M, Zhang ZQ, Wu M. Zha YY, et al. Zhonghua Zhong Liu Za Zhi. 2003 Jul;25(4):320-4. Zhonghua Zhong Liu Za Zhi. 2003. PMID: 12921556 Chinese.
METHODS: pcDNA-neu transfected into B16F10 with transfection reagent Fugene 6, neu-overexpressed cell clone B16F10-neu was selected with limited dilution method. ...
METHODS: pcDNA-neu transfected into B16F10 with transfection reagent Fugene 6, neu-overexpressed cell clone B16F10-neu was selected w …
[Comparison of the relative luciferase activity in secondary CEF by different heterogenous strong promoters, MDV gB promoter and the composed promoters].
Qiu YF, Ge FF, Xu XQ, Chen PY. Qiu YF, et al. Wei Sheng Wu Xue Bao. 2006 Apr;46(2):314-7. Wei Sheng Wu Xue Bao. 2006. PMID: 16736599 Chinese.
In vitro, these vectors and internal standard plasmid (pSV-beta-LacZ) were transiently co-transfected into secondary CEF by FuGene 6 Transfection Reagent. Furthermore, cells were harvested 48 hours after transfection. ...
In vitro, these vectors and internal standard plasmid (pSV-beta-LacZ) were transiently co-transfected into secondary CEF by FuGene 6 …